Marker Gene Technologies, Inc

Marker Gene Technology, Inc.

Ying Jiang

John Joseph Naleway

Daniel J Coleman

Gabriele M Cook

Ryan Link-Cole

The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration.

Reagents and methods for direct labeling of nucleotides

A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from(Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.

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Eugene, OR, United States of America

Ying Jiang