Ciba-geigy Corporation

Novartis Finance Corporation

Syngenta Participations Ag

Novartis Corporation

Basf Plant Science Company Gmbh

Yin-Fu Chang

Christian T Harms

Gleta Carswell

Ray Shillito

Mary-Dell Matchett Chilton

Andrea Itano

James Richard Wong

Stephen J Mejza

Leslie Walker

Weining Gu

Heng Zhong

Erik M Dunder

Eric Boudreau

Paula Olhoft

Leslie Grist

Cindy Boyce

Holly Tuttle

Sabrina Rouse

Aparna Sri Vanguri

Hai Ping Hong

Erik Martin Dunder

Sabrina Noel Rouse

The present invention relates to an improved method of producing a transgenic plant. Said method comprises, inter alia, the steps of a) providing a wounded transformable explant comprising a hypocotyl or a portion thereof, at least one cotyledon and wounded tissue, b) transforming cells comprised by said explant, and c) transferring said explant to a growing medium, comprising at least one selection compound for a selectable marker, by inserting the hypocotyl of said explant into said growing medium. Moreover, the present invention relates to a plant obtainable by the method according to the present invention.

Plant transformation method

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash,and pepper.

Methods for stable transformation of plants

The present invention is drawn to the production of fertile transformed wheat plants. The method involved subjecting wheat tissues to high velocity microprojectile bombardment, selecting for transformed cells, and regenerating stably transformed fertile plants from the transformed cells.

Methods for stable transformation of wheat

According to the invention there is provided a method of controlling insect larvae comprising the steps of feeding said larvae an insecticidal amount of a transgenic Zea mays plant that expresses a polypeptide having the insect toxicity properties of Bacillus thuringiensis crystal protein.

Method of controlling insect larvae comprising feeding an insecticidal

Methods of regenerating fertile Zea mays plants from protoplasts or protoplast-derived cells are described. The protoplasts or cells may be derived from embryogenic cell cultures or callus cultures. The protoplasts, cells and resulting plants may be transgenic, containing, for example, chimeric genes coding for a polypeptide having substantially the insect toxicity properties of the crystal protein produced by Bacillus thuringiensis.

Zea mays plants regenerated from protoplasts or protoplast-derived cells

Method of regenerating fertile transgenic Zea mays plants from

Methods for protecting ZEA mays plants against pest damage

Zea mays plants and transgenic Zea mays plants regenerated from

Growing community of inventors

Carrboro, NC, United States of America

Yin-Fu Chang