Cellomics, Inc.

Prolume, Ltd.

Yih-Tai Chen

Longguang Cao

Gary L Bright

Daniel Rajadavid Premkumar

The present invention provides novel recombinant fusion proteins for detecting binding of a molecule of interest containing a detection domain, a first and optionally a second localization domain, and a binding domain. The invention also provides recombinant nucleic acid molecules and recombinant expression vectors encoding these novel fusion proteins, genetically engineered host cells containing these expression vectors, and kits for the use of these fusion proteins, nucleic acid molecules, expression vectors, and host cells. Additionally, the present invention provides methods for identifying compounds that alter the binding of a molecule of interest in a cell.

Fusion proteins and assays for molecular binding

Synthetic versions of a full length and termini truncated humanized green fluorescent protein based onare disclosed which have been modified to the favored or most favored codons for mammalian expression systems. The disclosed encoded protein has 239 amino acid residues compared with the wild typewhich has 238 amino acids. In the present invention, a valine residue has been added at the second position from the amino terminus and codon preference bias has been changed in a majority of the wild type codons offluorescent protein. The humanizedgreen fluorescent protein is useful as a fluorescent tag for monitoring the activities of its fusion partners using imaging based approaches.

Synthetic DNA encoding an orange seapen-derived green fluorescent protein with codon preference of mammalian expression systems and biosensors

Synthetic versions of a full length and termini truncated humanized green fluorescent protein based on are disclosed which have been modified to the favored or most favored codons for mammalian expression systems. The disclosed encoded protein has 239 amino acid residues compared with the wild type which has 238 amino acids. In the present invention, a valine residue has been added at the second position from the amino terminus and codon preference bias has been changed in a majority of the wild type codons of fluorescent protein. The humanized green fluorescent protein is useful as a fluorescent tag for monitoring the activities of its fusion partners using imaging based approaches.

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Gibsonia, PA, United States of America

Yih-Tai Chen