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Emory University

Yih-Ling Tzeng

David S Stephens

Anup K Datta

Russell W Carlson

Susu Zughaier

Seshu K Gudlavalleti

Shanta Zimmer

Provided are methods for recombinant production of an O-acetyltransferase and methods for acetylating capsular polysaccharides, especially those of a Serogroup Ausing the recombinant O-acetyltransferase, and immunogenic compositions comprising the acetylated capsular polysaccharide.

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Provided herein are mutant strains ofwhich produce Kdo-free lipid A as well as the Kdo-free lipid A molecules and immunogenic compositions containing such Kdo-free lipid A molecules from astrain containing a genetically stable mutation which inactivates a gene selected from the group consisting of genes encoding arabinose-5-phosphate isomerase, CMP-Kdo synthetase and CMP-Kdo transferase.NMB206 is a specifically exemplified strain which harbors a stable insertion mutation in the gene (kpsF) encoding A5P isomerase; strain NMB-249 is a specifically exemplified strain with a stable insertion mutation in the gene (kdtA) encoding CMP-Kdo synthetase, and strain NMB259 is specifically exemplified strain with a stable insertion mutation in the gene (kdsB) encoding CMP-Kdo transferase. Also provided by the present invention are methods for the production of Lipid A flee of 3-keto-3-deoxyoctanoic acid using these genetically stablemutants. Also describes is pYT250, a plasmid functional in neisseriae and in enterics such as

Neisseria mutants, lipooligosaccharides and immunogenic compositions

Growing community of inventors

Atlanta, GA, United States of America

Yih-Ling Tzeng