Linden Technologies, Inc.

Yih Huang

Tai-Nang Huang

Ming Shen

Simon W Law

Shin-Yih Chen

Amy Anderson Deik

Haisun Liao

Natalia Mamaeva

Caroline Ngaara Woodward

Disclosed is an insoluble support that can be used, for example, for producing replicates of sample nucleic acids. The support includes a plurality of attached oligonucleotides that include a prokaryotic promoter sequence and a target annealing sequence 3' of the promote. The proximal end of the promoter sequence is spaced from the insoluble support by a distance greater than 10 nm.

Nucleic acid amplification

Immobilized nucleotide primers of this invention include a modified nucleotide tethered to a support substrate through a linking group. In particular, the modified nucleotide is constructed such that the C-5&prime; end of the nucleotide is tetherable to the linking group and the protected C-3&prime; end is available for further controlled modification, e.g., addition of other nucleotides in specific sequences to the immobilized nucleotide primer. Additionally, the linking group is of sufficient length to allow the immobilized nucleotide primer to be used to synthesize and screen arrays of oligonucleotides, e.g., enzymatic C-3&prime; primer extension.

C-3&prime; protected monomeric nucleotides and synthesis of oligonucleotides on solid support

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Lexington, MA, United States of America

Yih Huang