10x Genomics, Inc.

Yifeng Yin

Benjamin J Hindson

Andrew D Price

Michael Schnall-Levin

Rajiv Bharadwaj

Tobias Daniel Wheeler

Zahra Kamila Belhocine

Jennifer Chew

Katherine Pfeiffer

Eswar Prasad Ramachandran Iyer

Cedric Uytingco

Christopher Hindson

Pranav Patel

Anthony Makarewicz

Michael Ybarra Lucero

David Sukovich

Mohammad Rahimi Lenji

Christina Galonska

Luz Montesclaros

James Michael Chell

Jerald Sapida

Augusto Manuel Tentori

Tarjei Sigurd Mikkelsen

Marlon Stoeckius

Bill Kengli Lin

Zachary Bent

Josephine Lee

Francesca Meschi

Zeljko Dzakula

Joakim Lundeberg

Layla Katiraee

Hanyoup Kim

Kamila Belhocine

Steven W Short

Felice Alessio Bava

Neil Ira Weisenfeld

Kevin J Wu

Vijay Kumar Sreenivasa Gopalan

Jeffrey Clark Mellen

Preyas Shah

David Maurice Cox

Jasper Staab

David Michael Patterson

Joseph Francis Shuga

Albert Dale Kim

Shea Thompson Lance

Brynn Claypoole

Linda Kvastad

Narek Dshkhunyan

Stephen R Williams

Dulce Ovando Morales

Andrej Hartnett

Patrick Roelli

Ryo Hatori

Rapolas Spalinskas

Mesruh Turkekul

Anna-Maria Katsori

Aleksandra Jurek

Octavian Marian Bloju

Alexander Hermes

Denise Cheung

Naishitha Anaparthy

Meghan L F Frey

Fernandino Valdecanas

Erik Leonard Henrik Borgstrom

Stefania Giacomello

Alexander Gagnon

Eileen Dalin

Sultan Doganay Tuncer

Benjamin McCreath

Nabil Mikhaiel

Eva Gracia Villacampa

Ian Fiddes

Florian Baumgartner

The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

Methods and systems for sample preparation and analysis

Provided herein are methods for capturing an analyte from a first region of interest of a biological sample on a substrate, where the biological sample comprises the first region of interest and a second region, and where the method includes contacting the second region with a sealant in order to create a hydrophobic seal thereby preventing an interaction between an analyte from the second region with a capture domain of a capture probe.

Methods for increasing resolution of spatial analysis

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Methods and systems for analysis

Methods and systems for droplet-based single cell barcoding

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

Imaging system hardware

Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

Multiplex capture of gene and protein expression from a biological sample

The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

Generating capture probes for spatial analysis

Provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample using splint oligonucleotides, circularized second strands, and rolling circle amplification. For example, provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample where a splint oligonucleotide hybridizes to a second strand; the second strand is ligated together thereby creating a circularized second strand, rolling circle amplification of the circularized second strand results in generation of an amplified second strand, and all or part of the sequence of the amplified second strand is determined and used to spatially detect the analyte in the biological sample.

Methods for spatial analysis using rolling circle amplification and detection probes

Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Spatially-tagged analyte capture agents for analyte multiplexing

Systems and methods for inferring a status of a cell population are provided. Described techniques allow deconvolving a first clonal population comprising a first plurality of cells of a species, wherein nucleic acid sequence reads from each cell in the first plurality of cells are obtained. The nucleic acid sequence reads are mapped into bins representing portions of a reference genome, and a pattern of sequence read counts for each cell across the multiple bins is used to assign a cell to a group, thereby inferring a mitotic status of the cell. The assignment of nucleic acid sequence reads into bins is also be used for segregating cells into classes based on a status of a certain biological marker in each cell. Comparison of sequence read counts for a subset of bins across the cell classes allows evaluating effect of a compound on a cell status.

Systems and methods for inferring cell status

Provided herein are methods of determining a location of a target analyte in a non-permeabilized biological sample that include the use of a blocking probe.

Methods for determining a location of an analyte in a biological sample

Growing community of inventors

Elk Grove, CA, United States of America

Yifeng Yin