Mgi Tech Co., Ltd.

Yifan Xie

Lin Yang

Hui Fu Jiang

Fang Zhou Chen

Yanyan Zhang

Yuqian Wang

Ya Gao

Haiping Zhang

Haojun Jiang

Guodong Huang

Shaohong Zhang

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3' of the first primer, and the first random sequence is located on end 5&#x2032; of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3&#x2032; of the second primer, and the second random sequence is located on end 5&#x2032; of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.

PCR primer pair and application thereof

Growing community of inventors

Shenzhen, China

Yifan Xie