University of California

Leland Stanford Junior University

Aarhus University

Yifan Cheng

James David Marks

Jianlong Lou

Jody Lynn Baron

Stephen L Nishimura

Anthony Cormier

Shenping Wu

Naoki Takasaka

Saburo Ito

Melody G Campbell

Feng Wang

Eric Andrew Appel

David A Agard

Anton Smith

Henriette E Autzen

Eugene Palovcak

Provided herein are compositions including lipids and copolymers in the form of a nanodisc assembly. The subject copolymers include monomer units of styrene and monomer units selected from acrylic acid and an acrylic acid derivative. In certain cases, the copolymer is a copolymer of styrene and acrylic acid. Also provided herein, is an aqueous solution comprising the subject composition. Also provided herein, are methods for producing a nanodisc assembly, including incubation of a lipid and a subject copolymer. Further provided herein, are methods for solubilizing a membrane protein in an aqueous solution, wherein the method includes forming a nanodisc assembly of a lipid bilayer having one or more membrane proteins embedded therein, and a subject copolymer. Also provided are methods of solubilizing a hydrophobic constituent in an aqueous solution, including forming a nanodisc assembly of a lipid, a hydrophobic constituent, and a subject copolymer.

Lipid nanodiscs solubilized through poly(acrylic acid-co-styrene) copolymers

Herein are innovations that enable facile cryo-EM analysis of diverse samples. Methods of functionalizing sample grids for cryo-EM are described, including methods of creating high quality graphene oxide films on cryo-EM substrates. The cryo-EM sample substrates are functionalized with affinity molecules that efficiently concentrate sample molecules and other specimen types on the grid, away from the air-water interface. Affinity groups include amines and proteins such as tagging system proteins and peptides that can be used to capture diverse sample types with high affinity. Optionally, spacers such as PEG chains are used to place sample particles away from the substrate surface, reducing substrate-induced artifacts.

Graphene oxide affinity sample grids for Cyro-EM

Antibodies that bind to αvβ8 are provided.

Antibodies that bind integrin αvβ8 and uses thereof

New antibodies and methods of use are described.

Neutralizing antibodies to the αvβ8 integrin complex for immunotherapy

Antibodies that bind to &#x3b1;&#x3bd;&#x3b2;8 are provided.

Antibodies that bind integrin AVB8 and uses thereof

Neutralizing antibodies to the alpha v beta 8 integrin complex for immunotherapy

Provided is an antibody that specifically binds human &#x3b1;v&#x3b2;&#x3b2; and blocks binding of TGFp peptide to &#x3b1;v&#x3b2;8, wherein the antibody binds to the specificity determining loop (SDL) of human &#x3b2;8. In some embodiments, the antibody further binds to one, two, or all three of the human av-head domain, the al helix of human &#x3b2;8, or the al helix of human &#x3b2;8. In some embodiments, the antibody is humanized or chimeric. In some embodiments, the antibody is linked to a detectable label. Also provided is a method of enhancing an immune response in a human individual, comprising administering a sufficient amount of the antibody to the individual, thereby enhancing an immune response. Also provided are pharmaceutical compositions comprising the anti-&#x3b1;v&#x3b2;&#x3b2; antibodies or antigen-binding molecules thereof.

Neutralizing antibodies to the &#x3b1;v&#x3b2;8 integrin complex for immunotherapy

Growing community of inventors

San Francisco, CA, United States of America

Yifan Cheng