Anaspec, Inc.

Yi Tang

Zhenjun Diwu

Jianheng Zhang

Xiang Guobing

Jianjun He

The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.

Luminescence quenching compounds

Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3'-position, their bioconjugates and their uses are described. 1,3&#x2032;-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1&#x2032;-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Kits for analysis of biological samples

Fluorescent dyes

The present invention relates generally to the detection and measurement of transmembrane potentials using an N,N,N'-trialkyl thiobarbituric acid-derived polymethine oxonol (shown below). In particular, the present invention is directed to compositions and optical methods for determining transmembrane potentials across the plasma membrane of biological cells using a slightly hydrophobic N,N,N&#x2032;-trialkyl thiobarbituric acid-derived polymethine oxonols. The method comprises a slightly hydrophobic N,N,N&#x2032;-trialkyl thiobarbituric acid-derived polymethine oxonol anion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes. wherein R1, R2, and R3 are independently selected from the group consisting of hydrogen, alkyl, haloalkyl and heteroalkyl; n is an integer from 1 to 3; Z is Na, K, ammonium or other biologically acceptable salt.

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Sunnyvale, CA, United States of America

Yi Tang