Food Industry Research and Development Institute

Yi-Pei Chen

Gwo-Fang Yuan

Li-Ling Liaw

Ching-Ping Tseng

Chun-Lin Wang

Ing-Er Hwang

Chung-Tsai Lee

Cholesterol inhibitor produced by, monacolin k, is a secondary metabolite of polyketides. The invention provides probes specific to monacolin k biosynthesis gene cluster. BAC clones having putative monacolin k gene cluster were screened from BAC (bacterial artificial chromosome) library, and sequencing and annotation were performed on these clones. The results show that 2 polyketide synthase (PKS) genes and 7 regulatory genes related to monacolin k synthesis were obtained. Full-length cDNAs of these genes were then obtained by RT-PCR and cloned to expression vectors for the expression of these genes.

Monacolin K biosynthesis genes

Retrotransposons and methods related thereto. The retrotransposon comprises a nucleotide sequence selected from a group consisting of a nucleotide sequence of SEQ ID NO: 1, a nucleotide sequence of SEQ ID NO: 2, a nucleotide sequence of SEQ ID NO: 3, and a nucleotide sequence encoding a polypeptide of an amino acid sequence of SEQ ID NO: 4.

Fungi non-LTR retrotransposons and methods related thereto

Growing community of inventors

Yingge, Taiwan

Yi-Pei Chen