10x Genomics, Inc.

Yi Luo

Felice Alessio Bava

Adam Lowe

Meiliana Tjandra

Shalini Gohil

Joshua Delaney

David Michael Patterson

Albert Dale Kim

Kristen Pham

Monica Nagendran

Hong Chen

Veronica Emelina Gonzalez Muñoz

Joshua Gu

Tathagata Mukherjee

James Francis Perna, Iii

Michael John Terry Stubbington

Wyatt James Mcdonnell

The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.

Catalytic de-crosslinking of samples for in situ analysis

The present disclosure generally relates to methods and compositions for in situ analysis or detection of analytes in a biological sample. More specifically, the present disclosure relates to methods for reducing autofluorescence in tissue samples, methods for analyzing tissue samples, and compounds for use in the same. The methods and compounds of the present disclosure may be especially suitable for analytical methods employing fluorescence in situ hybridization techniques over multiple cycles of imaging.

Compositions and methods for targeted masking of autofluorescence

Provided herein are methods of sequencing comprising click chemistry bioconjugation. In some embodiments, target polynucleotide sequences on the same or different molecules are contacted with and hybridize to probes comprising click functional groups. Probes (e.g., reading probes) hybridizing to adaptor sequences adjacent to different sequences of interest (e.g., barcodes to be sequenced) can be hybridized simultaneously in large pools. In some embodiments, the provided methods achieve multiplexing without requiring separate hybridization of probes (e.g., reading probes) for each sequencing-by-ligation cycle, thereby reducing total hybridization time which is typically a most time-consuming step in in situ technologies. In some aspects, the hybridized probes (e.g., reading probes) are clicked onto detectable probes to analyze a sequence of a target polynucleotide in a sequencing-by-ligation fashion.

Nucleic acid assays using click chemistry bioconjugation

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Dublin, CA, United States of America

Yi Luo