Cognis Corporation

Yeyan Zhang

C Ron Wilson

David L Craft

Dudley Eirich

Jeffrey B Stavenhagen

The present invention provides modified promoters fromCYP and POX4 genes. The modified promoters have various sequence motifs added, deleted, or altered in order to modulate expression of a coding sequence operably linked thereto. The sequence motifs comprise repressors of gene induction (URS sequences) and activators of gene induction (UAS sequences) as well as oleic acid response elements (ORE sequences). Yeast host cells comprising such modified promoters are also provided. Methods of altering expression of a protein of the beta or omega oxidation pathways using a subject modified promoter are also provided.

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The present invention provides antigenic peptides useful for the production of antibodies which selectively bind to an enzyme involved in the &#x3c9;-oxidation of fatty acids and alkanes to &#x3b1;,&#x3c9;-dicarboxylic acids in yeast. Antibodies which specifically bind to an enzyme involved in the &#x3c9;-oxidation of fatty acids and alkanes to &#x3b1;,&#x3c9;-dicarboxylic acids in yeast are also provided. In addition, methods of producing the subject antibodies, a method of detecting the presence and amount of a specific enzyme involved in the &#x3c9;-oxidation of fatty acids and alkanes to &#x3b1;,&#x3c9;-dicarboxylic acids, and a method of monitoring the degree of enzyme induction and/or enzyme stability in a mixture during &#x3c9;-oxidation of fatty acids and alkanes to &#x3b1;,&#x3c9;-dicarboxylic acids in yeast, are also provided.

Antibodies for enzymes of the &#x3c9;-oxidation pathway and methods relating thereto

A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.

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Mason, OH, United States of America

Yeyan Zhang