Other

Chaoyang University of Technology

Kaiyew Investment Corp.

Jenisa Biotechnology Corp.

Deyew Biotech Corp.

Yew-Min Tzeng

Chi-Tai Yeh

Zhen Yang

Hang Shi

Cheng-Ming Liu

Dar-Fu Tai

Tuo-Ping Luo

Venkatachalam Angamuthu

Chuang-Chuang Li

Tien-Shen Yang

The present invention provides a method for preparing antrocin of pharmaceutically acceptable salts thereof via a series of gold-catalyzed cyclization to construct the (6-6-5) tricyclic core frame. The present invention also provides a use of a composition in preparing drugs for suppressing growth of non-small cell lung cancer cells, wherein the composition comprises an effective amount of antrocin or pharmaceutically acceptable salts thereof and a pharmaceutically acceptable carrier.

Method for chemical synthesis of antrocin and use thereof for suppressing non-small cell lung cancer

The present invention relates to a process of preparation of optically active antrocin. At first, to introduce the correct configuration of trans-decalone, alkyl aluminum/TMSCN was used to react with decalenone. The resulting racemic nitrile-decalone was resolved with chiral diol by ketalization to produce two chromatography separable diasteromers. After a simple column chromatography, optically pure compounds were obtained. Formylation was a critical step for the lactone formation. The rest of the synthesis is straight forward through oxidation and olefination. Accordingly, the total synthesis was completed in 10 steps with 7% overall yield from commercially available 6-methoxy-2-tetralone.

Process for preparing optically active antrocin

This subject invention is directed to a method for inhibition of cancer cells, comprising administrating an effective amount of a compound of formula I (Sesquiterpene lactones, antrocin) or pharmaceutically acceptable salts thereof, together with a pharmaceutically acceptable carrier, wherein the cancer cells are selected from colorectal cancer cells, liver cancer cells, lung cancer cells or breast cancer cells.

Antrocin containing pharmaceutical compositions for inhibiting cancer cells

Quantitative analysis for thuringiensin by capillary electrophoresis (&ldquo;CE') was demonstrated. CE is a suitable separation technique for thuringiensin because of its high resolution, great efficiency, rapid analysis and small consumption of sample. Tryptophan, an aromatic amino acid sharing a similar UV absorptive spectrum as thuringiensin, was used as an internal standard to avoid some inaccurate results caused by the questionable stability of purified thuringiensin. A mixed amount of tryptophan was mixed with varied amount of newly made thuringiensin standards for CE analysis. The electropherograms showed that the peak of tryptophan appeared around 8 minutes, which is 6 minutes earlier than the peak of thuringiensin. The peak area ratio between thuringiensin and tryptophan is proportional to the amount of thuringiensin in the mixture. The linear regression has been established to assess the peak area ratio that can be used to quantify the concentration of thuringiensin. This method provides a faster and more accurate quantitative analysis than the traditional methods.

Method of quantitative analysis for thuringiensin by capillary electrophoresis

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Taichung, Taiwan

Yew-Min Tzeng