Agency for Science, Technology and Research

Yeon Joon Park

Jackie Yi-Ru Ying

Min-Han Tan

Igor Cima

Yoke San Daniel Lee

Wai Min Phyo

Jackie Y Ying

A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.

Column-based device for retrieval of rare cells based on size, and uses thereof

Column-based device and method for retrieval of rare cells based on size, and uses thereof

Growing community of inventors

Singapore, Singapore

Yeon Joon Park