Industrial Technology Research Institute

Academia Sinica

Yen-Ju Lin

Min-Yuan Chou

Cheng-Tao Wu

Fu-Tong Liu

Mei-Wei Lin

Chung-Cheng Liu

Jui-Wen Huang

Wei-Chen Hsieh

Huan-Yuan Chen

A method for screening host cells expressing a target protein is provided. The method includes the following steps: providing an expression vector, the expression vector including a promoter, a gene encoding a target protein and an FTH1 gene; transfecting the host cells with the expression vector; culturing the host cells in a medium; and adding iron ions to the medium, and screening the surviving host cells to obtain the host cells expressing the target protein. An expression vector and a method for establishing a cell line stably expressing an exogenous recombinant gene are also provided.

Methods for screening host cells expressing target proteins

A small interfering RNA is provided. The small interfering RNA consists of a passenger strand and a guide strand, wherein the sequence of the passenger strand comprises the sequence of SEQ ID NO. 7, and the sequence of the guide strand comprises the sequence of SEQ ID NO. 8, wherein the small interfering RNA is capable of inhibiting galectin-12 expression.

Small interfering RNA and pharmaceutical composition and method for inhibiting galectin-12 expression and/or enhancing lipolysis containing the same

Provided herein is a method for detection of migratory and invasive cancer cells based on a number of marker nucleic acids differentially expressed in migratory/invasive cancer cells relative to nonmigratory/noninvasive cancer cells. Also disclosed are antisense oligonucleotides of the marker nucleic acids and uses thereof for suppressing cancer cell migration and invasion.

Oligonucleotides for suppressing cancer cell invasion and migration

Growing community of inventors

Taichung, Taiwan

Yen-Ju Lin