Berkeley Lights, Inc.

Bruker Cellular Analysis, Inc.

Bruker Cellular Analysic, Inc.

Yelena Bronevetsky

Peter J Beemiller

Kevin T Chapman

Xiaohua Wang

Guido K Stadler

Alexander J Mastroianni

Randall D Lowe, Jr

Kristin G Beaumont

Gregory G Lavieu

Xiao Guan Radstrom

Natalie C Marks

Annamaria Mocciaro

Thomas M Vetterli

Hansohl E Kim

Vincent Haw Tien Pai

Kevin D Loutherback

Jason M Mcewen

Amanda L Goodsell

John A Tenney

Duane Smith

Jr Randall D Lowe

Andrew W Mcfarland

Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

Methods for assaying biological cells in a microfluidic device

In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces of wells within a well plate to activate lymphocytes, including but not limited to T lymphocytes, in a controllable and reproducible manner.

Functionalized well plate, methods of preparation and use thereof

Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (D) that separates activated T lymphocytes from na&#xef;ve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from na&#xef;ve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

Sorting of T lymphocytes in a microfluidic device

The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.

Tumor infiltrating cells engineered to express a pro-inflammatory polypeptide

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Alameda, CA, United States of America

Yelena Bronevetsky