E.i. Dupont De Nemours and Company

Nutrition & Biosciences USA 4, Inc.

Dupont Industrial Biosciences Usa, LLC

C H Erbsloh Kg

Covation, Inc.

Yefim Brun

Mark Scott Payne

Thomas Scholz

Hongxian He

Jayme L Paullin

Rakesh Nambiar

Rong Guan

James Joshua Ohane

Robert DiCosimo

Richard Ray Bott

Qiong Cheng

T Joseph Dennes

Yuanfeng Liang

Laurie A Howe

Charles R Powley

Lucia Daniela Ionescu

Kyle Hyun Chang Kim

Nikita Iltchenko

Lemuel Tong

Jorge Mok

Juan David Londono

Susan Marie Hennessey

Yougen Li

Kevin D Nagy

Michael Reichman

Brian McCauley

Natnael Behabtu

Karlheinz Hausmann

Elizabeth Forrester McCord

Fredrik Nederberg

David Mark Scott

Michael D Gagnon

Joseph F Roeske

Alicia Marie Castagna

Alicia Maria Castagna

Ken-Hsuan Liao

Christopher John Rasmussen

Joerg Wallach

Elizabeth Forrester Mccord

Disclosed herein are compositions comprising insoluble alpha-glucan particles having a high degree of crystallinity and small particle size. For example, the alpha-glucan particles can have a degree of crystallinity of at least about 0.65, and/or an average size of less than a micron. At least 50% of the glycosidic linkages of the insoluble alpha-glucan in the disclosed particles are alpha-1,3 glycosidic linkages. Further disclosed are methods of producing insoluble alpha-glucan particles, as well as their use in various applications and products.

Highly crystalline alpha-1,3-glucan

Compositions are disclosed herein comprising dextran that comprises (i) 87-93 wt % glucose linked at positions 1 and 6; (ii) 0.1-1.2 wt % glucose linked at positions 1 and 3; (iii) 0.1-0.7 wt % glucose linked at positions 1 and 4; (iv) 7.7-8.6 wt % glucose linked at positions 1, 3 and 6; and (v) about 0.4-1.7 wt % glucose linked at (a) positions 1, 2 and 6, or (b) positions 1, 4 and 6. Aqueous forms of this composition have enhanced viscosity profiles. Further disclosed are methods of using compositions comprising dextran, such as increasing the viscosity of an aqueous composition. Enzymatic reactions for producing dextran are also disclosed.

Enzymatically polymerized gelling dextrans

Compositions are disclosed herein comprising a graft copolymer that comprises: (i) a backbone comprising dextran that has been modified with about 1%-25% alpha-1,2 branches, and (ii) one or more alpha-1,3-glucan side chains comprising at least about 50% alpha-1,3 glycosidic linkages. Further disclosed are reactions for producing such graft copolymers, as well as their use in derivatives, films and various other applications.

Alpha-1,3-glucan graft copolymers

Compositions are disclosed herein comprising a graft copolymer that comprises: (i) a backbone comprising an alpha-1,3-glucan ether or ester compound, and (ii) one or more alpha-1,3-glucan side chains comprising at least about 50% alpha-1,3 glycosidic linkages. Further disclosed are reactions for producing such graft copolymers, as well as their use in various applications.

A method for producing insoluble alpha-1,3-glucan is disclosed. Embodiments of the method comprise providing (i) oligosaccharides that comprise alpha-1,3 and alpha-1,6 glycosidic linkages, or (ii) oligosaccharides derived from a glucosyltransferase reaction; and contacting at least water, sucrose, a glucosyltransferase enzyme, and the oligosaccharides provided in the first step. Glucosyltransferase reaction compositions embodying such a method, and insoluble products thereof, are also disclosed. Yield and other product benefits can be realized when practicing the disclosed subject matter.

Enzymatic production of alpha-1,3-glucan

Nonwoven webs comprising a water insoluble &#x3b1;-(1,3&#x2192;glucan) polymer and methods of forming the nonwoven webs are disclosed. The water insoluble &#x3b1;-(1,3&#x2192;glucan) polymer comprises 90% or greater &#x3b1;-1,3-glycosidic linkages, less than 1% by weight of &#x3b1;-1,3,6-glycosidic branch points, a number average degree of polymerization in the range of from 55 to 10,000, and a ratio of apparent DPw to true DPw in the range of from 2 to 10. The nonwoven webs can be used for personal hygiene wipes, filtration media, apparel, or other uses.

Nonwoven glucan webs

Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.

Glucosyltransferase enzymes for production of glucan polymers

Compositions are disclosed herein comprising poly alpha-1,3-1,6-glucan with a weight average degree of polymerization (DP) of at least 1000. This glucan polymer comprises at least 30% alpha-1,3 linkages and at least 30% alpha-1,6 linkages. Further disclosed are glucosyltransferase enzymes that synthesize poly alpha-1,3-1,6-glucan. Ether derivatives of poly alpha-1,3-1,6-glucan and methods of using such derivatives as viscosity modifiers are also disclosed.

Reactions comprising water, sucrose, and one or more glucosyltransferase enzymes are disclosed herein. Glucosyltransferase enzymes used in these reactions comprise certain motifs allowing production of insoluble poly alpha-1,3-glucan having at least 95% alpha-1,3 glycosidic linkages.

Glucosyltransferase amino acid motifs for enzymatic production of linear poly alpha-1,3-glucan

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Wilmington, DE, United States of America

Yefim Brun