Tianjin University

Asymchem Life Science (Tianjin) Co., Ltd

Asymchem Laboratories (Fuxin) Co., Ltd.

Ye Liu

Hao Hong

Na Zhang

Gage James

Fang Liu

Junjie Yan

Zujian Wang

Yi Xiao

Yuan Bi

Mujiao Zhang

Gang Lv

Wanming Shi

Chonglin Song

An amino acid sequence of the transaminase mutant is an amino acid sequence obtained by a mutation of an amino acid sequence is shown in SEQ ID NO: 1. The mutation occurred at least one of the following mutation sites: G17V, L36P, Q40H, G69Y, H70T, L73A, V77G, V77S, V77T, A78I, Y130M, Y130V, Y130T, N132I, N132T, K141S, K142S, K142T, R143P, G144F, G144W, G144Y, E145D, E145S, E145G, K146R, L148A, L148I and the like.

Transaminase mutant and use thereof

Provided are a transaminase mutant and a method for producing a chiral amine using the same. An amino acid sequence of the transaminase mutant is an amino acid sequence obtained by mutation occurred in an amino acid sequence as shown in SEQ ID NO: 1, and the mutation includes at least one of the following mutation sites: position 3, position 5, position 8, position 25, position 32, position 45, position 56, position 59, position 60, position 84, position 86, position 164, position 176, position 178, position 180, position 187, position 197, position 206, position 207, position 242, position 245, position 319 and position 324.

The present disclosure discloses a method for detecting electrical characteristics of individual soot nanoparticles, wherein the electrical characteristics comprise conductance and work function. The conductance of individual soot nanoparticles is measured by a PF-TUNA mode of an atomic force microscope, and the work function of soot nanoparticles is measured by a KPFM mode of the atomic force microscope. The method mainly comprises steps of preparing a gold film substrate, sampling soot nanoparticles, measuring the conductance of individual soot nanoparticles, and measuring the work function of soot nanoparticles. The detection method of the present disclosure reduces the influence on the inherent characteristics of soot nanoparticles.

Method for detecting electrical characteristics of individual soot nanoparticles and application thereof

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Tianjin, China

Ye Liu