Zhejiang University

Enzymaster (Ningbo) Bio-Engineering Co., Ltd.

Shenzhen Grandstream Networks Technologies Co., Ltd

Grandstream Networks, Inc.

Ye Li

Li Chen

Haibin Chen

Xi Chen

Jun Huang

Qisong Ding

Fangfang Fan

Linka Yu

Yifeng Zhou

Xiabin Li

Chunyan Liu

Lehe Mei

Hongpeng Wang

Kepan Gao

Xiaofeng Xu

Changjiang Lv

Sheng Hu

Bi Chen

Weirui Zhao

The present invention discloses a ω-transaminase mutant obtained through DNA synthetic shuffling combined mutation. The ω-transaminase mutant is obtained through point mutation of a wild type ω-transaminase from. The amino acid sequence of the wild type ω-transaminase is shown in SEQ ID NO: 1. The mutation site of the ω-transaminase mutant is any one of: (1) F115L-H210N-M150C-M280C; (2) F115L-H210N; (3) F115L-H210N-E253A-I295V; (4) I77L-F115L-E133A-H210N-N245D; (5) I77L-Q97E-F115L-L118T-E253A-G292D; (6) I77L-E133A-N245D-G292D; and (7) H210N-N245D-E253A-G292D. According to the present invention, forward mutations obtained in the previous stage are randomly combined through a DNA synthetic shuffling combined mutation method. It is verified through experiments that this method can effectively improve the probability of forward mutation and increase experimental efficiency and feasibility, and is capable of obtaining mutant enzymes with thermodynamic stability remarkably superior to that of wild enzymes via screening.

ω-Transaminase mutant obtained by DNA synthetic shuffling combined mutation and use

The present invention provides a conference processing method of a third-party application, capable of connecting a call of the third-party application to a teleconference of the communication device. The method includes the following steps: receiving, by a virtual sound card/virtual graphic card, data from a third-party application; and if a teleconference exists in the system, further judging whether the third-party application is allowed to join in the teleconference; wherein if it is judged that the third-party application is allowed to join in the teleconference, a sound mixing module and a screen mixing module mix audio and video data transmitted over at least one local session line and audio and video data of the third-party application, and complete sound mixing playing and mixing and superposition display; and otherwise, the audio and video data of the third-party application is shielded.

Conference processing method of third-party application and communication device thereof

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Hangzhou, China

Ye Li