Yale University

Other

Yatindra Prashar

Sherman M Weissman

Jon Goguen

Peter E Newburger

Subrahmanyam V Yerramilli

The present invention is directed to an approach to identify changes in gene expression by selective amplification of 3&prime; fragments of double stranded cDNAs.

Analysis of gene expression by display of 3'-end fragments of CDNAS

The present invention comprises a method to identify granulocytic cell genes that are differentially expressed upon exposure to a pathogen or in a sterile inflammatory disease by preparing a gene expression profile of a granulocytic cell population exposed to a pathogen or isolated from a subject having a sterile inflammatory disease and comparing that profile to a profile prepared from quiescent granulocytic cells. The present invention is particularly useful for identifying cytokine genes, genes encoding cell surface receptors and genes encoding intermediary signaling molecules. The invention also includes methods to identify a therapeutic agent that modulates the expression of at least one gene in a granulocytic population. Genes which are differentially expressed during neutrophil contact with a pathogen, such as a virulent bacteria, or that are differentially expressed in a subject having a sterile inflammatory disease are of particular importance.

Process to study changes in gene expression in granulocytic cells

The present invention is directed to an approach to study changes in gene expression by the selective PCR amplification and display of 3'-end restriction fragments of double stranded cDNAs.

Analysis of gene expression by display of 3'-end restriction fragments

The present invention provides methods to analyze gene expression by selective PCR amplification and display of 3'-end restriction fragments of double stranded cDNAs.

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Columbia, MD, United States of America

Yatindra Prashar