Kyushu University

Hiroshima University

Yasuyuki Okawa

Takashi Yamamoto

Tetsushi Sakuma

Yusuke Yagi

Takahiro Nakamura

The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an &#x3b1;-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an &#x3b1;-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. 'ii' (&#x2212;2) A. A. contained in L of the formula 1, the aforementioned object could be achieved.(Helix A)-X-(Helix B)-L&#x2003;&#x2003;(Formula 1)

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Fukuoka, Japan

Yasuyuki Okawa