Sysmex Corporation

Yasuyuki Kawashima

Junya Inoue

Yasuhiro Sakai

Yoshiro Ikeuchi

Masatsugu Ozasa

Yousuke Tanaka

Kazuhiro Yamada

Seiichiro Tabata

Keiko Yoshikawa

Masayuki Ikeda

Yusuke Konishi

A blood cell analyzer comprises a flow cell configured to flow a measurement specimen containing blood cells, a first light source configured to emit light having a first wavelength, a second light source configured to emit light having a second wavelength different from the first wavelength, a first light receiving portion configured to receive first scattered light obtained by irradiating the blood cells passing through the flow cell with light from the first light source, a second light receiving portion configured to receive second scattered light obtained by irradiating the blood cells passing through the flow cell with light from the second light source, and a control section configured to discriminate at least red blood cells from the blood cells contained in the measurement specimen based on detection signals output from the first light receiving portion and the second light receiving portion, respectively.

Blood cell analyzer and blood cell analyzing method

An apparatus for measuring bacteria includes a sampling device for processing fluorescently stained bacteria, a detector for detecting size information from the bacteria in the sample, and a detector for detecting fluorescence information expressing intensity of fluorescent light emitted by the bacteria. The apparatus further includes a processor and memory that includes programs for creating a scattergram representing a distribution of the bacteria based on the size information and the fluorescence information detected, analyzing the distribution of the bacteria in the scattergram, and determining whether the bacteria in the sample isorbased on a result of the analysis.

Bacteria measuring apparatuses

An apparatus, intended for use in analyzing particles in urine is disclosed, that comprising: a sample distribution section for distributing urine samples to a first aliquot and a second aliquot; a first specimen preparing section for preparing a first specimen for measuring urinary particles, containing at least erythrocytes, by mixing a first stain reagent and the first aliquot; a second specimen preparing section for preparing a second specimen for measuring bacteria by mixing a second stain reagent and the second aliquot; and an optical detecting section comprising a light source for irradiating a light to a specimen being supplied, a scattered light receiving element for detecting scattered light emitted from the specimen, and a fluorescence receiving element for detecting fluorescence emitted from the specimen. A method intended for use in analyzing particles in urine is also disclosed.

Apparatus for analyzing particles in urine and method thereof

An apparatus for measuring bacteria that includes a sampling device for processing fluorescently stained bacteria, a detector for detecting size information from the bacteria in the sample, and a detector for detecting fluorescence information expressing intensity of fluorescent light emitted by the bacteria. The apparatus further includes a processor and memory that includes programs for creating a scattergram representing a distribution of the bacteria based on the size information and the fluorescence information detected, analyzing the distribution of the bacteria in the scattergram, and determining whether the bacteria in the sample isorbased on a result of the analysis.

An apparatus for analyzing bacteria is described that includes an analytic sample preparation section for preparing an analytic sample by treating a specimen so as to generate a morphological difference between Gram-negative bacteria and Gram-positive bacteria, a detector for detecting optical information from each particle contained in the analytic sample and an analyzing section for detecting Gram-positive bacteria contained on the basis of the detected optical information. A method for analyzing bacteria is also described.

Apparatus and method for analyzing bacteria

A reagent for analyzing urine is described. The reagent comprises a fungus membrane damaging agent for damaging a cellular membrane of yeast-like fungus in urine; a first dye for staining yeast-like fungus so that a fluorescent intensity of damaged yeast-like fungus becomes more intense than that of erythrocyte in urine; and a second dye for staining sperm in urine so that a fluorescent intensity of sperm becomes more intense than that of the damaged yeast-like fungus.

Reagent for analyzing urine and method for analyzing urine

Sample analyzers for analyzing a sample are described that include a pipette for suctioning the sample; a sample preparation unit for preparing a measured sample by diluting the sample supplied by the pipette with an acidic solution; a pipette washing unit for washing the pipette with the acidic solution; a detection unit for obtaining a detection signal from the measured sample prepared by the sample preparation unit; and a controller for calculating an analysis result from the detection signal obtained by the detection unit. Bacteria analyzers for analyzing bacteria and solutions for use in sample analyzers are also described.

Sample analyzers, bacteria analyzers, and solutions for diluting and cleaning

A method of staining bacteria comprises: working a polymethine dye on a sample in the presence of a substance capable of reducing nitrite ions to stain bacteria in the sample. A method of detecting bacteria comprises the following steps of: (1) working a polymethine dye on a sample by a method as described above to stain bacteria in the sample, (2) introducing the thus treated sample into a detecting part of a flow cytometer and irradiating cells of the stained bacteria one by one with light to measure scattered light and fluorescent light emitted from each of the cells; and (3) discriminating the bacteria from other components in accordance with an intensity of a scattered light signal and an intensity of a fluorescent light signal or a pulse width reflecting the length of particles to count the bacteria.

Method of preparing assay sample for discriminating bacteria by flow cytometer

A method for distinguishing erythrocytes in a biological specimen, includes preparing a sample liquid so as to cause damage to a cell membrane of yeast-like fungi without hemolyzing erythrocytes in a biological specimen and to stain the yeast-like fungi with a fluorescent dye; detecting a first information and a second information from a particle in the sample liquid, wherein the first information reflects a size of the particle and the second information reflects a degree of fluorescent staining of the particle; and distinguishing the erythrocytes from the yeast-like fungi based on the first information and second information detected. An apparatus, a reagent kit and a reagent for carrying out the method are also disclosed.

Method, apparatus, reagent kit and reagent for distinguishing erythrocytes in a biological specimen

An apparatus for analyzing bacteria is described that includes an analyte sample preparing section for preparing an analyte sample from a specimen; a detector for detecting optical information from each particle in the analyte sample; and a controller for detecting non-fermentative bacteria on the basis of the detected optical information. A method for analyzing bacteria is also described.

Methods for counting bacteria are described that include: (a) preparing an assay sample by staining a specimen using a fluorescent dye, thereby producing a difference in fluorescent intensity between live bacteria and dead bacteria; (b) detecting optical information from the assay sample; and (c) classifying and counting the live bacteria and the dead bacteria based on the detected optical information. Bacteria counting apparatuses and reagent kits for counting bacteria are also described.

Growing community of inventors

Kobe, Japan

Yasuyuki Kawashima