The Biomembrane Institute

Oncomembrane, Inc.

Otsuka America Pharmaceutical, Inc.

Yasuyuki Igarashi

Sen-Itiroh Hakomori

Sen-itiroh Hakomori

Hirofumi Okoshi

Shigeyuki Kawa

Yoshito Sadahira

Fuqiang Ruan

Mahammad N Ahmad

Edward D Nudelman

Steven B Levery

Kazuko Handa

Fugiang Ruan

Mohammad N Ahmad

Khalid Sadozai

Hideki Ohta

Yutaka Yatomi

Yong S Park

Satoshi Kimura

Sen-itroh Hakomori

The invention relates to trimethylsphingosine derivatives which have some or all of the activities of the parent compound.

Trimethylsphingosine derivatives

A method of inhibiting tumor cell chemotactic motility and/or chemoinvasion, a method of inhibiting phagokinetic activity of tumor cells and neutrophils, a method of inhibiting tumor cell metastasis and a method of inhibiting inflammation due to motility and invasion into blood vessel walls of neutrophils comprising contacting the cells or administering to a host in need of treatment an inhibitory amount of an agent selected from the group consisting of sphingosine-1-phosphate, derivatives of sphingosine-1-phosphate, and mimetics of the sphingosine-1-phosphate or of the derivatives, and pharmaceutically acceptable salts of the agent. A method of preparing sphingosine-1-phosphate and its derivatives is also described.

Method for inhibition of cell motility by sphingosine-1-phosphate and

An isolated plasmalopsychosine selected from the group consisting of compound A and compound B: ##STR1## wherein n1 is 0-50 or an isolated synthetic plasmalocerebroside selected from the group consisting of compound C and compound D: ##STR2## wherein n2 and n3 each is 0-50.

Plasmalopsychosines and plasmalocerebrosides

A method of detecting sphingosines and platelet activation is disclosed.

Method for quantifying sphingosine and for diagnosing platelet activation

A method of inhibiting tumor cell chemotactic and/or chemoinvasion motility comprising contacting the tumor cell with an inhibitory amount of an agent selected from the group consisting of sphingosine-1-phosphate, derivatives of sphingosine-1-phosphate and mimetics of the sphingosine-1-phosphate or of the derivatives. A method of inhibiting phagokinetic activity of tumor cells and neutrophils comprising contacting the cells with a phagokinetic inhibitory amount of an agent selected from the group consisting of sphingosine-1-phosphate, derivatives of sphingosine-1-phosphate, and mimetics of the sphingosine-1-phosphate or of the derivatives. A method of inhibiting tumor cell metastasis comprising administering to a host in need of treatment a metastasis inhibitory amount of an agent selected from the group consisting of sphingosine-1-phosphate, derivatives of sphingosine-1-phosphate, and mimetics of the sphingosine-1-phosphate or of the derivatives, and pharmaceutically acceptable salts of the agent. A method of inhibiting inflammation due to motility and invasion into blood vessel walls of neutrophils comprising administering to a host in need of treatment an inflammation inhibitory amount of an agent selected from the group consisting of sphingosine-1-phosphate, derivatives of sphingosine-1-phosphate, and mimetics of the sphingosine-1-phosphate or of the derivatives, and pharmaceutically acceptable salts of the agent. A method of preparing sphingosine-1-phosphate and its derivatives.

Sphingosine-1-phosphate essentially free of L-threo isomer

Sphingosine-1-phosphate/N,N,N-trimethylsphingosine composition to modulate cell growth.

Sphingosine-1-phosphate/trimethylsphingosine composition

N-methylated sphingosine used to induce apoptosis.

Methylsphingosine used to treat apoptosis

Liposomal N,N,N-Trimethylsphingosine and pharmaceutical compositions comprising same.

Liposomal trimethylsphingosine

Method for inhibition of cell motility by sphingosine-1-phosphate, its

Effect of N,N,N-trimethylsphingosine on protein kinase-C activity;

The invention relates to a novel compound, compositions and medicaments thereof and a method of inhibiting cell proliferation, platelet aggregation (induced by various factors), and inhibiting malignant phenotypes of tumor cells such as those having a metastatic property, using said compound, composition or medicament. N,N,N-trimethylsphingosine shows superior cell proliferation inhibitory and anti-metastatic activity over related compounds.

Method of preparing N,N,N-trimethylsphingosine

The invention relates to a novel compound, compositions and medicaments thereof and a method of inhibiting cell proliferation, platelet aggregation (induced by various factors) and inhibiting malignant phenotypes of tumor cells such as those having a metastatic property, and modulating cell adhesion molecule expression and function using said compound, composition or medicament. N,N,N-trimethylsphingosine shows superior cell proliferation inhibitory an anti-metastatic activity over related compounds.

Growing community of inventors

Seattle, WA, United States of America

Yasuyuki Igarashi