Kao Corporation

Yasushi Takimura

Nobuyuki Sumitomo

Tsuyoshi Sato

Mitsuyoshi Okuda

Tohru Kobayashi

Fumikazu Takahashi

Masafumi Nomura

Hiroshi Yoshida

Kazuyuki Fukuda

Takahiro Asada

Yoshifumi Izawa

To provide a method for supplying lauric acid with algae. A method for producing an oil or fat containing lauric acid as a constituent fatty acid including: culturing, in a medium, at least one species of algae in the class Cryptophyceae selected from the group consisting of algae belonging to the genusand algae belonging to the genusselected from among, andand recovering, from the culture product, an oil or fat having a lauric acid content of 3 weight % or higher of the fatty acid composition.

Method of producing lauric acid-containing oil or fat

To provide a method for supplying lauric acid with algae. The method for producing an oil or fat containing lauric acid as a constituent fatty acid includes culturing algae in the class Chlorarachniophyceae in a medium and recovering, from the culture product, an oil or fat having a lauric acid content of 3 weight % or higher of the fatty acid composition.

Method of producing lauric acid or an ester thereof

To provide a plasmid vector having high productivity which can be used for large scale production of an industrially valuable and useful protein, and a transformant using the plasmid vector. A plasmid vector having DNA which encodes a plasmid replication protein in which one or more amino acid residues that are selected from (a) position 48, (b) position 262, (c) position 149 and (d) position 198 in the amino acid sequence represented by SEQ ID NO: 2 are substituted with (a) Ala, Gly, Thr, Arg, Glu, Asn or Gln, (b) Gly, Ser, Thr, Cys or Val, (c) Asn, and (d) Glu, respectively.

Vector encoding a plasmid replication protein and use thereof

A novel musk-based fragrance having an excellent musky fragrance is provided. Also provided is a fragrance composition containing, as an active ingredient, a macrocyclic lactone compound represented by the following formula (1): wherein A represents an ethylene group or an ethenylene group; and when A is an ethylene group, R represents an alkyl group having 3 carbon atoms, and n represents an integer from 1 to 6, while when A is an ethenylene group, R represents an alkyl group having 1 to 3 carbon atoms, and n represents an integer from 1 to 6. 

Fragrance composition

The present invention provides an alkaline protease exhibiting high detergency and productivity, which is obtained by further enhancing the detergency of an alkaline protease effective against complex dirt and enhancing the specific activity.

Alkaline protease

An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ No; 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d): valine, (e): serine, (f): arginine, and (g); and a gene encoding the alkaline protease.

The present invention relates to genes expressing an alkaline protease having a prepro sequence, vectors containing the same, and transformants containing said vector. The present invention also relates to a method for producing an alkaline protease.

Nucleic acid sequences encoding modified prepro peptides of an alkaline protease

The present invention relates to an alkaline protease having a prepro sequence, wherein the prepro sequence is a mutant sequence of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 80% or higher homology to the amino acid sequence of SEQ ID NO: 1, in which amino acid residues at (a) position 52, (b) position 75, and (c) position 142 of SEQ ID NO: 1, or amino acid residues at positions corresponding to these positions are substituted by the following amino acid residues: According to the present invention, an alkaline protease increased in production can be produced. In particular, there can be efficiently produced an alkaline protease having an activity even in the presence of a fatty acid of high concentration and exhibiting excellent detergency against complex soils containing proteins and sebum.

Growing community of inventors

Haga-gun, Japan

Yasushi Takimura