Chugai Seiyaku Kabushiki Kaisha

Yasushi Shimonaka

Hideki Adachi

Naohiro Yabuta

Kunihiro Hattori

A method for measuring the presence or absence and/or strength of the inhibitory activity of a test substance on binding between a ligand and a receptor thereof, which comprises the following steps (1) to (3): (1) immobilizing either of the test substance or the ligand on a solid support; (2) contacting the test substance and the ligand for a given period of time in the presence or absence of the receptor for the ligand; and (3) comparing the level of binding between the test substance and the ligand in the presence and absence of the receptor.

Method for measuring inhibitory activity on ligand-receptor binding

In the case when there are two objective biological activities, and the aim is to isolate a compound having at least one biological activity, the present inventors developed an assay method wherein a common detection marker is utilized for separately detecting the presence or absence of each of the biological activities. The present inventors discovered that a compound having at least one of two or more distinct biological activities can be efficiently and conveniently detected by simultaneously assaying at least one test sample or more by the above-mentioned method. Furthermore, for a test sample that proved to be positive by the detection method, they found that it is possible to efficiently and conveniently screen for a test sample having an objective specific biological activity by combining with a method wherein an individual activity of a test sample can be detected to specify the biological activity.

Screening methods for biologically active ligands

It has been revealed that human interleukin-15 (hIL-15) acts on the megakaryocyte-thrombocyte system to accelerate its differentiation, maturation and/or proliferation, thereby accelerating the formation of thrombocytes. The present invention provides a treatment using IL-15 for thrombocytopenia and diseases accompanied by thrombocyte dysfunctions.

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Kanagawa, Japan

Yasushi Shimonaka