Takara Bio Inc.

Yasushi Katayama

Ikunoshin Kato

Junichi Mineno

Kiyozo Asada

Kazutoh Takesako

Hideto Chono

Hirofumi Yoshioka

Kazuya Matsumoto

Hiromi Okuyama

Masao Funakoshi

Daisuke Tomura

A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.

Method of retrovirus storage

A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a state equivalent to that in a serum-containing medium to produce a virus vector at a sufficient titer. The virus vector prepared from the virus producer cell cultivated in the medium exhibits gene transfer efficiency comparable to that of a conventional vector. The medium is useful for gene therapy and studies thereof.

Serum-free medium for producing retroviruses

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Shiga, Japan

Yasushi Katayama