Institute for Antibodies Co., Ltd.

Other

Medical Biological Laboratories Co., Ltd.

Yasushi Akahori

Yoshikazu Kurosawa

Yoshitaka Iba

Nobuhiro Takahashi

Kazuhiro Suzuki

Keiko Ogawa

Gene Kurosawa

Kimiyasu Shiraki

Yoshinobu Okuno

Kazuhiro Morishita

Yoshinori Ukai

Motohide Takahashi

Atsushi Sugioka

Mariko Sumitomo

Sari Fujiyama

Masachika Azuma

Kazuhiko Morino

Midori Shinohara

Nobuhiro Hayashi

Akihiko Takasaki

Miwa Morita

Susumu Tsustumi

Kazuki Matsuda

Chiho Muramatsu

Toshio Honda

An antibody library is prepared by selecting a light chain variable region capable of binding to the variable region of heavy chain to reproduce an active conformation and using the same. Because of being capable of maintaining the diversity of the heavy chain variable region at a high ratio in vitro, the antibody library of the present invention is expected as enabling the acquisition of antibodies with various binding activities.

Antibody library

It is intended to clarify a molecule which is available as a target in treating or diagnosing cancer and utilize the molecule in the medical field or the research field. By treating IgSF4, which has been identified as a molecule specifically expressed in lung cancer cells, with an antibody, and ADCC activity is exerted. Based on this finding, an anti-IgSF4 antibody is provided as a means efficacious in treating cancer, etc.

Anti-IgSF4 antibody and utilization of the same

The present invention provides camel antibody libraries that maintain in vivo diversity of camelid antibody variable region genes. The in vivo diversity of antibody variable region genes can be accomplished by, for example, mixing genes derived from a plurality of animals or modifying gene amplification conditions. Conventional methods yield only VHHs with limited repertoire diversity. However, the present invention provides libraries comprising genes encoding functional VHHs with sufficient repertoire size. According to the present invention, libraries that enable to freely obtain VHHs against arbitrary antigens are provided. VHHs have excellent solubility and stability, and show a reactivity that usually cannot be expected from tetrameric IgGs.

Methods of constructing camel antibody libraries

Growing community of inventors

Nagoya, Japan

Yasushi Akahori