Hokkaido University

Sumitomo Bakelite Company Limited

Shionogi Company Limited

Cellseed, Inc.

Yasuro Shinohara

Yoshiaki Miura

Shinichiro Nishimura

Jun-ichi Furukawa

Hideyuki Shimaoka

Yoko Kita

Hiroshi Yamazaki

Shin-Ichiro Nishimura

Hideaki Sakai

Mika Nakano

Akio Takimoto

Hiromitsu Kuramoto

Michio Horiuchi

Toshiharu Kuroda

Hiroaki Motoki

Hiroaki Sugiyama

Naoki Fujitani

Jun-Ichi Furukawa

Furukawa Jun-Ichi

The present invention provides a method for preparing a sample characterized by binding a substance A containing a hydrazide group to a sugar chain and/or a sugar derivative via hydrazone formation between the hydrazide group of the substance A and the reducing end of the sugar chain and/or the sugar derivative thereby to enable the separation and purification of the sugar chain and/or the sugar derivative for an analytical sample from a biological sample containing the sugar chain and/or the sugar derivative by a simple operation.

Sugar chain-capturing substance and use thereof

To provide an autoanalyzer for analyzing a sugar chain contained in a biological sample, in particular, serum. Namely, it is intended to provide a method of analyzing a sugar chain in a sample, which comprises the following steps: A) the sugar chain-releasing step of releasing the sugar chain in the sample; B) the detection sample-preparing step of preparing the released sugar chain for detection; and, in the case of conducting mass spectrometry using a plate, C) the step of forming a plate for the mass spectrometry having the captured sugar chain dotted thereon which comprises the step of providing the tagged sugar chain sample solution obtained in the step B) on a collection plate; and, if required, the step of conducting an operation in a solid phase support-enclosed plate to form the plate for mass spectrometry; and D) the step of analyzing the sugar chain to be assayed.

Apparatus for auto-pretreating sugar chain

A means for pre-treatment in glycomic analysis of a glycoprotein is provided by the present invention. A salt of the general formula (I): wherein Z, X, R, R, M, m and n are the same as described in DESCRIPTION,is useful as a protein solubilizer, and an oligosaccharide is efficiently released from a sample if reductive alkylation and/or digestion by a proteinase are carried out under the presence of the said solubilizer at the first step in the glycomic analysis of glycoprotein derived from a living body.

Method of serum pre-treatment for glycomic analysis

A method for preparing an analysis sample which involves the sugar chain capture step comprising a reaction of capturing a sugar chain and/or a sugar derivative from a biological sample by using a sugar chain capture agent and the excision step comprising excising a compound containing a moiety capturing the sugar chain and/or the sugar derivative from the sugar chain capture agent after the completion of the sugar chain capture reaction and releasing the compound.

Method for preparing analysis sample, analysis sample and sugar chain capture agent

Growing community of inventors

Hokkaido, Japan

Yasuro Shinohara