Sapporo Breweries Limited

Yasuo Yoneta

Hisao Kado

Yutaka Mitani

Suguru Takeo

Nobuhiro Watanabe

Makoto Hidaka

Takeshi Uozumi

Akira Nakamura

Michihisa Maeda

Haruhiko Masaki

A polysaccharide RON substance which has antitumor activity can be produced by fermenting sucrose with a synthetase. The synthetase can be isolated from Leuconostoc strains. Preferred strains of the microorganism are Leuconostoc mesenteroides FERM BP-2242, 2711, 2712, 2713, 2714, and 2670. The substance exhibits antitumor activity against syngenic tumor Meth A, a transplantable tumor in mice.

Process for producing polysaccharide ron substance with a synthetase

A biologically active polysaccharide RON substance which is excellent in such biological activities as antitumor activities, is produced by using a microorganism belonging to the genus Leuconostoc, which has an activity of producing the RON substance.

Method for producing biologically active polysaccharide RON substance

An isolated structural Bacillus sp. TB-90 (FERM BP-795) urease gene, which comprises base sequences encoding the amino acid sequences of three subunits of urease. A recombinant DNA comprising Bacillus sp. TB-90 (FERM BP-795) urease gene capable of replicating in Escherichia coli. A process for producing urease, which comprises cultivating Escherichia coli carrying a recombinant DNA comprising Bacillus sp. TB-90 (FERM BP-795) urease gene capable of replicating in Escherichia coli and recovering urease from the culture mixture

Gene and process for producing a thermostable urease

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Yaizu, Japan

Yasuo Yoneta