Toray Industries, Inc.

Yasunobu Wada

Katsushige Yamada

Hiroyuki Kurihara

Kazuhiko Ishikawa

Yuji Kado

Shiomi Watanabe

Yumiko Mishima

A &#x3b2;-glucosidase exhibits high activity in the presence of biomass and has high thermal stability compared to conventional enzymes. The &#x3b2;-glucosidase includes substitutions and/or deletions of amino acids, at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent &#x3b2;-glucosidase with other amino acids and exhibits a decomposition activity.

Mutant &#x3b2;-glucosidase, enzyme composition for decomposing biomass, and method of producing sugar solution

A method for producing a mutant glucosidase includes introducing DNA encoding a secretion signal sequence and DNA encoding Asn-X-Ser or Asn-X-Thr into DNA encoding a glucosidase derived from a thermophile, and introducing the resulting DNA into a eukaryotic microorganism and expressing it as a secretory protein. An enzyme composition contains the mutant glucosidase.

Method for producing glucosidase, enzyme composition, and method for hydrolyzing biomass

Growing community of inventors

Osaka, Japan

Yasunobu Wada