Kao Corporation

Yasunao Wada

Tohru Kobayashi

Susumu Ito

Keiji Endo

Mitsuyoshi Okuda

Masaki Tsumadori

Katsuhisa Saeki

Kenzo Koike

Masatoshi Tohata

Shitsuw Shikata

Yuji Hatada

Kazuhisa Sawada

Yoshifumi Izawa

Yoshihiro Hakamada

Hiroshi Kodama

Shitsu Shikata

Yumi Nishimura

Shitsuu Shikata

Miyuki Kasai

Atsushi Suzumatsu

Takato Kawano

Providing an alkaline protease exhibiting an improved solubility in a liquid detergent. A mutant alkaline protease consisting of the amino acid sequence represented by SEQ ID No: 2 or an amino acid sequence having 80% or more sequence identity therewith, in which at least one amino acid residue selected from the group consisting of the amino acid residues at predetermined positions of the amino acid sequence represented by SEQ ID No: 2 or corresponding positions thereto are substituted.

Improving the solubility of an alkaline protease in a liquid detergent by amino acid substitution

The present invention relates to alkaline cellulase variants having an amino acid sequence which exhibits at least 95% homology with the amino acid sequence of SEQ ID NO: 2, wherein said alkaline cellulase variant is mutated to include at least one substitution at (a) position 10, (b) position 16, (c) position 22, (d) position 33, (e) position 39, (f) position 76, (g) position 109, (h) position 242, (i) position 263, (j) position 308, (k) position 462, (l) position 466, (m) position 468, (n) position 552, (o) position 564, or (p) position 608 in SEQ ID NO: 2, or at a position corresponding thereto, wherein said alkaline cellulase variant has alkaline cellulase activity. The present invention also relates to a gene encoding the variant; a vector containing the gene; a transformant containing the vector; and a detergent composition containing the alkaline cellulase variant.

Alkaline cellulase variants

Detergent compositions containing protopectinase whose optimum pH for the reaction is 7.0 or higher when protopectin or polygalacturonic acid is used as a substrate. The detergent compositions are endowed with remarkable detergency against mud soil.

Detergent composition

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Wakayama, Japan

Yasunao Wada