Aisin Seiki Kabushiki Kaisha

Kabushiki Kaisha Toyota Chuo Kenkyusho

Yasumitsu Kondoh

Satoshi Fujita

Naoto Kagiyama

Masayoshi Momiyama

Osamu Asami

Yukio Yamada

Hidehiko Sugiyama

Takekazu Yamamoto

Paidi Yella Reddy

Takeshi Toru

Miho Nishiyauchi

Hiroko Uchiyama

A fluorescent dye-forming composition is provided that contains a fluorescence-generating substrate of formula (I):

Composition containing fluorescence-generating substrate

A method of detecting nucleic acids, proteins, or protein nucleic acid complexes. The method includes binding an enzyme, such as phosphatase, to a specimen of the nucleic acid, protein, or protein nucleic acid complex. The enzyme is then reacted with a fluorescein derivative phosphate ester to obtain a fluorescein derivative phosphate ester hydrolysate. The hydrolysate is then irradiated with excitation light, and the emitted fluorescein is detected.

Method of detecting nucleic acids or the like

A method of detecting nucleic acid utilizes hybridization between the nucleic acid to be detected which is present on an immobilizing support and a DNA probe which is complementary to at least a portion of the desired nucleotide sequence of the nucleic acid. The present DNA probe includes a hapten attached via a linker which a hapten is gibberellin or a gibberellin derivative. After hybridization by reaction between the nucleic acid to be detected and the DNA probe, the unreacted DNA probe is removed, and then labelled anti-hapten antibody is allowed to bind with the DNA probe of the hybrid and the labelling is used to detect the nucleic acid. Accordingly, stable detecting sensitivity may be achieved regardless of the label mixing ratio or labelling ratio, and thus regardless of the nucleotide sequence composition of the nucleic acid being tested.

Method of detecting nucleic acid

Growing community of inventors

Sapporo, Japan

Yasumitsu Kondoh