Kabushiki Kaisha Dnaform

Riken Corporation

K. K. Dnaform

Yasumasa Mitani

Yoshihide Hayashizaki

Takahiro Soma

Takeshi Hanami

Kengo Usui

Yasumasa Kimura

Yuki Kawai

Hajime Kanamori

Takahiro Arakawa

Takanori Oka

Masayoshi Itoh

Sotaro Uemura

Toshizo Hayashi

Akio Yamane

Yuko Shibata

The present invention is to provide a method for analyzing a target nucleic acid, by which the target nucleic acid can be analyzed rapidly and easily. In order to achieve the above object, the present invention provides a method for analyzing a target nucleic acid in a sample, including the step of: analyzing the target nucleic acid in the sample by bringing the sample into contact with a label and with a primer or probe that can hybridize to the target nucleic acid. The primer or probe is immobilized on a solid phase. The label does not emit light when the primer or probe does not hybridize to the target nucleic acid, whereas the label emits light when the primer or probe has hybridized to the target nucleic acid. The analysis is carried out by detecting the light emitted from the label.

Method for analyzing target nucleic acid, kit, and analyzer

The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.

Nucleic acid probe, method for designing nucleic acid probe, and method for detecting target sequence

A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3' end portion, a sequence (Ac&#x2032;) that hybridizes to a sequence (A) located in the 3&#x2032; end portion of the target nucleic acid sequence. The first primer also contains, on the 5&#x2032; side of the sequence (Ac&#x2032;), a sequence (B&#x2032;) that hybridizes to a complementary sequence (Bc) to a sequence (B) that is present on the 5&#x2032; side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3&#x2032; end portion, a sequence (Cc&#x2032;) that hybridizes to a sequence (C) located in the 3&#x2032; end portion of a complementary sequence to the target nucleic acid sequence. The second primer also contains, on the 5&#x2032; side of the sequence (Cc&#x2032;), a folded sequence (D-Dc&#x2032;) that contains, on the same strand, two nucleic acid sequences that hybridize to each other.

Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same

Problem to be solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively. Solution There is provided a method for detecting a nucleic acid contained in a sample, comprising the step of adding at least one substance selected from the group consisting of polyphenols, polyhydric alcohols, sugar acids, sugar alcohols, and hydrophilic biodegradable polymers to a sample, the step of complementarily binding an oligonucleotide complementary to a part of the nucleic acid sequence of a nucleic acid to be detected to a part of the nucleic acid sequence, and the step of detecting the nucleic acid to be detected.

Method for detecting and amplifying nucleic acid

The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. The process involves providing a primer comprising in its 3'-end portion a sequence (Ac&#x2032;) which hybridizes a sequence (A) in the 3&#x2032;-end portion of the target nucleic acid sequence, and in the 5&#x2032;-side of the sequence (Ac&#x2032;) a sequence (B&#x2032;) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5&#x2032;-side of the sequence (A) on the target nucleic acid sequence, wherein {X&#x2212;(Y&#x2212;Y&#x2032;)}/X is in the range of &#x2212;1.00 to 1.00, in which X denotes the number of bases in the sequence (Ac&#x2032;), Y denotes the number of bases in the region flanked by the sequences (A) and (B) in the target nucleic acid sequence, and Y&#x2032; denotes the number of bases in an intervening sequence between the sequences (Ac&#x2032;) and (B&#x2032;) (Y&#x2032; may be zero).

Process for amplifying nucleic acid

Growing community of inventors

Yokohama, Japan

Yasumasa Mitani