Kabushiki Kaisha Dnaform

Yasumasa Kimura

Yoshihide Hayashizaki

Yuki Tanaka

Takahiro Soma

Takeshi Hanami

Yasumasa Mitani

Kengo Usui

Yuki Kawai

Hajime Kanamori

The present invention is intended to provide a novel fluorescent labeled single-stranded nucleic acid, by which the background of an exciton oligomer can be further reduced and the novel use thereof. The present invention relates to a labeled single-stranded nucleic acid having at least two fluorescent atomic group pairs that exhibit an exciton effect. The labeled single-stranded nucleic acid is characterized in that the emission peak wavelength of one of the fluorescent atomic group pairs (fluorescent atomic group pair A) is shorter than the excitation peak wavelength of the other fluorescent atomic group pair (fluorescent atomic group pair B), and the fluorescent atomic group pairs A and B have a F&#xf6;rster resonance energy transfer (FRET) effect. This fluorescent labeled single-stranded nucleic acid is usable as a primer for amplifying a target nucleic acid or a probe to be hybridized with a target nucleic acid.

Fluorescent labeled single-stranded nucleic acid and use thereof

The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.

Nucleic acid probe, method for designing nucleic acid probe, and method for detecting target sequence

The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3' side thereof, a sequence (A&#x2032;) that can hybridize to a sequence (A) on the 3&#x2032; side of the target nucleic acid sequence. The second primer 1R includes, on the 3&#x2032; side thereof, a sequence (B&#x2032;) that can hybridize to a sequence (B) on the 3&#x2032; side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5&#x2032; sides thereof, sequences (C) that are substantially identical to each other.

Primer set for isothermal amplication of a target nucleic acid sequence

Growing community of inventors

Yokohama, Japan

Yasumasa Kimura