Sysmex Corporation

Yasumasa Akai

Yasunori Maekawa

Satoshi Nakayama

Shigeki Abe

Harumi Minekawa

Eiji Tanoshima

Yasuyuki Imura

Sachiyo Tada

Kadzuki Nakabayashi

Nobuaki Hanaoka

Sayaka Nakamura

Mikiko Nishizaki

A nucleic acid amplification apparatus for amplifying a target nucleic acid derived from living organism, comprising: a measurement unit for amplifying the target nucleic acid in a measuring sample prepared from the living organism, and measuring a product of the amplification of the target nucleic acid; a measurement value obtaining unit for obtaining a measurement value related to an amount of the product of the amplification; and a judging unit for judging whether amplification inhibition of the target nucleic acid occur or not based on a first measurement value and a second measurement value, the first measurement value obtained from a first measurement sample and the second measurement value obtained from a second measurement sample having a difference dilution ratio from the first measurement sample.

Nucleic acid amplification apparatus and method

It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.

Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers

Methods for detecting a malaria parasite are described that include: releasing the malaria parasite by allowing an erythrocyte in a specimen to undergo hemolysis; staining the released malaria parasite with a fluorescent dye to prepare a measurement sample; detecting optical information from the measurement sample; and distinguishing the malaria parasite based on the optical information obtained. Apparatuses and reagent kits are also described.

Methods for detecting malaria parasites and reagent kits therefor

An anticoagulant for blood cell counting containing at least two kinds of the following antiplatelet agents: theophyllines, adenosines and dipyridamoles. The anticoagulant also contains citric acid, an alkali metal salt thereof or a mixture of citric acid and an alkali metal salt thereof.

Anticoagulant and hemanalysis method

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Ono, Japan

Yasumasa Akai