Mitsui Chemicals, Inc.

Yasuko Matsuba

Toshihiro Oikawa

Kiyoteru Nagahara

Hiroki Ishibashi

Yasushi Fukuiri

Tadashi Araki

Nobuhiro Fukuhara

Kaori Matoishi

Ichirou Ikeda

Reiko Abe

The invention provides a method for producing a cytosine nucleoside compound from pentose-1-phosphate and cytosine or a derivative thereof using a nucleoside phosphorylase reactive to cytosine or a bacterium having the enzyme activity. The invention also provides a method for specifically reducing an activity to degrade the substrates or the product, resulting in efficient production of the cytosine nucleoside compound. According to the invention, little by-product is produced in producing cytonucleocide compounds.

Method for producing cytosine nucleoside compounds

A protected 2'-deoxycytidine is purified by precipitating the protected 2&#x2032;-deoxycytidine represented by general formula (3) in the form of a hydrated crystal from a solution containing the protected 2&#x2032;-deoxycytidine and water, and by recovering the protected 2&#x2032;-deoxycytidine:wherein R1 represents a 4-methoxytrityl, 4, 4&#x2032;-dimethoxytrityl, or triphenylmethyl group; and B1 represents a cytosine group having a protected amino group. The compound represented by general formula (3) is, in particular, a protected 2&#x2032;-deoxycytidine represented by formula (4):The 2&#x2032;-deoxycytidine is used as a raw material for antisense DNA.

Method for purifying protected 2'-deoxycytidines and hydrated crystals thereof

Optically active chroman-3-acetic acids and optically active chroman-3-acetic acid esters can be obtained by treating a mixture of (3R)- and (3S)-chroman-3-acetic esters of formula (I)

Esterase and methods for the production of optically active chroman compounds

Optically active chroman-3-acetic acids and optically active chroman-3-acetic acid esters can be obtained by treating a mixture of (3R)- and (3S)-chroman-3-acetic esters of formula (I) ##STR1## [wherein R.sub.1 is a straight or branched alkyl group having 1-5 carbon atoms and R.sub.2 is a hydrogen atom or substituted or unsubstituted amino group]

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Fukuoka, Japan

Yasuko Matsuba