Japan Science and Technology Agency

Yasuaki Kimura

Naoko Abe

Hiroshi Abe

Hiroki Murase

Kosuke Nakamoto

A method for producing a capped RNA which is an RNA having the 5′-end modified with a cap, the method including: reacting an activated capping compound represented by

Method for producing capped RNA

A primer for amplifying a nucleic acid having a structure represented by the formula (1): where B represents a base, Rrepresents a decomposable protecting group, and Rrepresents hydrogen or a hydroxyl group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA, includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3'-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; and a photoirradiation unit for deprotecting Rand forming a sticky end with a 3′-protruding end.

Primer, device for producing double-stranded DNA using primer, and method for producing double-stranded DNA using primer

where Arepresents —S—, —S—S—, or —Se—, B represents a base, and Rrepresents a decomposable protecting group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3'-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; Klenow fragment for making the 3′ ends blunt; and a photoirradiation unit for deprotecting Rand forming a sticky end with a 3′-protruding end.

A &#x3b2;-modified phosphoric acid compound precursor that inhibits the progress of a phosphorylation reaction having a partial structure represented by where Arepresents &#x2014;SR, &#x2014;S&#x2014;S&#x2014;R, &#x2014;SeR, or &#x2014;X, where X is a halogen selected from fluoro, chloro, bromo, and iodo; Rrepresents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; Lrepresents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; Lrepresents an alkyl group having 1 to 20 carbon atoms, or the like; Land Lmay be linked to each other to form a 4 to 6-membered ring structure; Land Lmay each have a substituent; and the symbol * represents a bond to be bonded to a phosphate group by phosphorylation. Further, provided are a reaction inhibitor and a medicine, each of which includes the &#x3b2;-modified phosphoric acid compound precursor.

&#x3b2;-modified phosphoric acid compound precursor, &#x3b2;-modified phosphoric acid compound, reaction inhibitor and medicine containing the same, and method for inhibiting reaction

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Nagoya, Japan

Yasuaki Kimura