Mgi Tech Co., Ltd.

Yaqiao Li

Radoje Drmanac

Hui Fu Jiang

Wenwei Zhang

Yuan Jiang

Chunyu Geng

Xia Zhao

Lingyu He

Andrei Alexeev

Rongrong Guo

Shujin Fu

Ruoying Chen

Xiaoshan Su

Fanzi Wu

Hui Jiang

Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3&#x2032; end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3&#x2032; end thereof can be in a complementary pairing with the 5&#x2032; end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

Linker element and method of using same to construct sequencing library

Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Method for constructing nucleic acid single-stranded cyclic library and reagents thereof

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Shenzhen, China

Yaqiao Li