Industrial Technology Research Institute

Yao-Sung Chang

Chao-Chi Pan

Cheng-Tao Wu

Jia-Huey Tsao

Chih-Wei Ho

Wen-Hsun Kuo

Zu-Sho Chow

Bor-Iuan Jan

Yu-Ching Liu

Yung-Chiang Chung

Ming-Zheng Shiu

Bor-Iaun Jan

A method and apparatus designed for nucleic acid hybridization employs a hydrogen bond denaturation area with a higher temperature and a lower temperature nucleic acid hybridization area that is immobilized with nucleic acid probes. Nucleic acid-containing samples are introduced into the hydrogen bond denaturation area for heating curled nucleic acids in samples so that they become linear and are guided into the nucleic acid hybridization area. In this area, the nucleic acid hybridization rate can be multiplied by increasing the kinetic energy by the repeated flow of driven fluid. The nucleic acid hybridization apparatus provided by the invention contains a hydrogen bond denaturation area, a nucleic acid hybridization area, a two-way driving apparatus, and a temperature control element. The temperatures in the hydrogen bond denaturation area and the nucleic acid hybridization area can be maintained through the management of this temperature control element, and the fluid can gain needed kinetic energy through the two-way driving pump.

Method for nucleic acid hybridization using back and forth flow between a denaturation channel and a hybridization channel via a connection channel

The invention discloses an on-spot hydrophilic enhanced slide/microarray. The preparation method relates to a hydrophobic copolymer prepared by blending, grafting or co-polymerization of a hydrophobic material and a compound bearing functional groups such as anhydride, imide, cyclic amide, and cyclic ester, and application of the hydrophobic copolymer onto an organic or inorganic substrate. The resulting slide has the properties of on-spot hydrophilic/hydrophobic dynamic conversion, as well as on-spot hydrophilic enhancement for the preparation of high-density and high-efficiency bio-chip/microarray.

On-spot hydrophilic enhanced slide and preparation thereof

The invention features a method for preparing a high-density functional slide by coating a sol-gel containing amine-group bearing silanes and a solution containing polyaldehyde groups onto an organic or inorganic substrate, respectively. The resulting slide is useful in the preparation of highly homogeneous functional-group slides and the high-density and high-efficiency bio-chip/microarray.

High-density functional slide and preparation method thereof

A method is provided for preparing an active slide, including introducing a monomer containing an aldehyde group, or a mixture of a monomer containing an aldehyde group and an acidic functional group provider into a plasma chamber; and depositing the aldehyde group and acidic functional group onto the surface of an organic or inorganic matrix using plasma deposition to form a slide comprising a layer of polymerized actively functional groups thereon. The aldehyde groups and negatively charged groups are deposited on the surface of the active slide, such that the bio-molecules bound thereto possess the properties of an inducible orientation and thus form a mono-layer.

Activated inorganic slide having aldehyde groups deposited by plasma deposition

Growing community of inventors

Hsinchu, Taiwan

Yao-Sung Chang