Cygnus Biosciences (beijing) Co., Ltd.

Peking University

Cygnus Biosciences Co. Ltd.

Yanyi Huang

Haifeng Duan

Li Kang

Shuo Qiao

Wenxiong Zhou

Zitian Chen

Fuchou Tang

Lu Wen

Jingyi Li

A method for sequencing a biological molecule, such as a nucleic acid molecule, and a method for detecting and/or correcting sequencing error(s) in the sequencing results are provided. Kits and systems based on the above methods are also provided.

Methods for obtaining and correcting biological sequence information

A high-throughput sequencing method for detecting methylated CpG islands includes: processing a DNA sample by using a modifier, and converting cytosine in the DNA sample into uracil, and keeping 5'methylcytosine unchanged; amplifying the obtained segment by using a primer A and DNA polymerase, to obtain a segment having one end being capable of anchoring a junction primer C; amplifying the obtained segment by using a primer B and DNA polymerase, to obtain a segment gathering methylated CpG islands and having two ends being capable of separately anchoring junction primers C and D; amplifying the obtained segment at a PCR exponent by using the junction primers C and D and the DNA polymerase, to obtain the amplified product; and separating and purifying the amplified product, to form a high-throughput sequencing library and perform computer sequencing, and data analysis.

High-throughput sequencing detection method for methylated CpG islands

Growing community of inventors

Beijing, China

Yanyi Huang