Dalian University of Technology

Dalian Chromas Bioscience Co., Ltd.

Dalian Firstar Information Materials Co., Ltd.

Yanxia He

Xiaojun Peng

Jiangli Fan

Shiguo Sun

Fengling Song

Zhigang Yang

Jingyun Wang

A pentamethine cyanine fluorescent dye having the general formula I, a synthetic method and a use thereof are disclosed. In the general formula, X is CHO or CHCRR; Rand Rare independently selected from the group consisting of (CH)Rand et al.; Rand Rare independently selected from the group consisting of CN, COOH and COOR; R, Rand Rare independently selected from the group consisting of H, SORand COOR; Ris H or Calkyl; Ris H or CH; Ris N(RRRR); Ris Calkyl; R, R, R, Rand Rare independently selected from the group consisting of H, Calkyl, (CH)ORand (CHRCHO)R; Yis halogen anion or OTs; and n, m and p are integers of 0-18. The dye can be utilized to sensitively detect viscosity of micro-environment such as tissues and cells. 

Pentamethine cyanine fluorescent dyes, preparation methods and uses thereof

A pentamethine cyanine fluorescent dye with N-substituting at &#x3b2;-position of conjugated chain is disclosed. The dye reacts with biosubstrate including protein, liposome and DNA, and shows excellent selectivity. It has good living cell membrane permeability and shows good selectivity in living cell staining In addition, the dye has good stability and low background fluorescence, and can be used together with other fluorescent dyes having consistent wavelengths for single-channel excitation and multi-channel detection. The dye can be used in the fields of protein labeling and detecting, fluorescence immunoassay and living cell selective imaging and the like.

Pentamethine cyanine fluorescent dye with N-substituting at &#x3b2;-position of conjugated chain

Growing community of inventors

Liaoning, China

Yanxia He