New England Biolabs, Inc.

Yanxia Bei

Shengxi Guan

Thomas C Evans, Jr

Nathan Tanner

Yinhua Zhang

Jennifer Ong

Nicole Nichols

Vladimir Potapov

Thomas C Evans

Variants of the bacteriophage B103 DNA polymerase are described herein. The variant has improved properties, that include when compared to wild-type Phi29 DNA polymerase, at least one of the following: increased thermostability, improved reaction rate for DNA amplification, reduced background and a reduction of bias. Methods of using the DNA polymerase variant are also described herein.

Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA

Compositions and methods for performing a template-switching reaction are provided that may include reducing or eliminating concatemerization of the template-switching oligonucleotide (TSO). In some embodiments, the composition may comprise: a reverse transcriptase; a TSO that includes a recognition sequence for a site-specific double strand nucleic acid cleaving enzyme, wherein the TSO has at its 3' end at least one nucleotide capable of hybridizing to at least one or more non-templated nucleotides added to a templated cDNA strand by the reverse transcriptase; and a site-specific double strand nucleic acid cleaving enzyme that cleaves the TSO at the recognition sequence.

Methods and compositions for preventing concatemerization during template-switching

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Ipswich, MA, United States of America

Yanxia Bei