Jiangsu Academy of Agricultural Sciences

Guotai (taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou

Yanna Wei

Li Wang

Zhixin Feng

Qiyan Xiong

Maojun Liu

Yuzi Wu

Zhanwei Wang

Yanfei Yu

Guoqing Shao

Beibei Liu

Yuan Gan

Jia Wang

Yun Bai

Haiyan Wang

Guoqinq Shao

Fangfang Bai

Daohua Zhang

Dongxia Liu

Lizhong Hua

A composition and a kit for detectingare provided. The composition for detectingis an aqueous solution including a primer M-F, a primer M-R, and a probe M-P. A sequence of the M-F is shown in SEQ ID NO: 1. A sequence of the M-R is shown in SEQ ID NO: 2. A nucleotide sequence of the probe M-P is shown in SEQ ID NO: 3, and includes a fluorophore FAM linked at a 5' terminus and a quencher BHQ1 linked at a 3′ terminus. The composition exhibits high sensitivity, strong specificity, and a wide detection range when used in the detection of

Composition and kit for detecting mycoplasma

Disclosed are an attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof. In the present invention, pathological lung tissues of swine having typical(Mhp) infection and no obvious other pathogenic infections are screened, and subcultured 100 generations in lungs of newborn rabbits; then, Mhp strains are isolated and serially subcultured in a medium; and the Mhp strain AN306 is obtained by screening a plurality of strains, which is deposited with an accession number: CCTCC NO. M2012431. Also disclosed is a live vaccine formulation against MPS prepared on the basis of the attenuated strain and comprising live attenuated strain, a pharmaceutically acceptable carrier or excipient, and optionally an adjuvant and immunogens of other pathogens.

Attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof

Growing community of inventors

Nanjing, China

Yanna Wei