Bio-rad Laboratories, Inc.

N6 Tec, Inc.

Yann Jouvenot

John Frederick Regan

Eli Hefner

Dianna Maar

Claudia Litterst

Svilen Tzonev

Geoff McDermott

Amir Sadri

Yassine Kabouzi

Serge Saxonov

Pranav Patel

Daniel Y Chu

Simant Dube

Samantha Cooper

Nick Heredia

Jen Berman

Viresh Patel

Svilen S Tzonev

Eli A Hefner

The present disclosure provides methods and systems comprising use of a device. A device may comprise a plurality of locations configured to contain one or more samples, a plurality of thermal elements corresponding and disposed adjacent to the plurality of locations, wherein the plurality of elements are configured to affect a thermal condition within individual locations of the plurality of locations, at least one additional thermal element operably coupled and common to at least a subset of the plurality of elements, wherein the additional thermal element is configured to affect at least one operating condition of the plurality of thermal elements at least partially in response to the thermal condition within the individual locations of the plurality of locations, and at least one optical system configured to detect a signal or change thereof from the individual locations of the plurality of locations, wherein the signal or change thereof is generated at least partially by a change of the thermal condition and is indicative of a property associated with the one or more samples.

Methods and systems for sample analysis

Compositions, kits, and methods are provided for the normalization of a quantitative polymerase chain reaction (PCR) amplification. Also provided are compositions, kits, and methods for multiplexing qPCR amplification of two or more target nucleic acids in the same well.

Quantitative amplification normalization with quenchers

Methods, compositions, and kits are provided for quantifying a number or frequency of double stranded breaks in the genome of a cell or in the genomes of a population of cells.

Detection of genome editing

System, including methods and apparatus, for performing a multiplexed digital assay.

Multiplexed digital assays

Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

Multiplexed digital assay with specific and generic reporters

An automated system captures multiple digital images of a sample, from different viewing areas of the sample. The images are analyzed and a test result may be reported based on the count of cells in all of the digital images. The system may be especially applicable to the testing of samples having low cell concentrations, as the variability between tests may be reduced as compared with a system that bases its test results on only a single image of the sample. The system may count the cells in a first digital image of the sample, and may capture or analyze one or more additional digital images only when it is recognized that additional measurements are needed to ensure adequate accuracy and repeatability of the test.

Cell counting systems and methods

Growing community of inventors

Benicia, CA, United States of America

Yann Jouvenot