Isis Innovation Limited

Oxford Biosencors Limited

F. Hoffmann-La Roche Ag

Yann Astier

Kevin Lorimer

Hagan Bayley

Orit Braha

John Hagan Bayley

Haichen Wu

Giovanni Maglia

Emma Naomi Kathleen Wallace-Davis

Emma Naomi Wallace-Davis

The invention relates to transmembrane protein pore for use in detecting a analyte in a sample. The pore comprises a molecular adaptor that facilitates an interaction between the pore and the analyte. The adaptor is covalently attached to the pore in an orientation that allows the analyte to be detected using the pore.

Molecular adaptors

The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.

Methods using pores

A method for determining the concentration of an analyte in a sample is disclosed which comprises contacting the sample with a micro electrode which comprises an enzyme capable of reacting with said analyte and a redox mediator which is capable of being converted by being oxidised or reduced by said enzyme once the latter has reacted with the analyte, allowing the analyte to react with the enzyme, then applying a potential across the electrode and measuring the resulting concentration of the converted mediator electrochemically.

Analyte measurement

Growing community of inventors

Oxford, United Kingdom

Yann Astier