Asymchem Laboratories (tianjin) Co., Ltd.

Jilin Asymchem Laboratories Co., Ltd.

Asymchem Laboratories (fuxin) Co., Ltd.

Tianjin University of Science and Technology

Asymchem Life Science (tianjin) Co., Ltd

Tianjin Asymchem Pharmaceuticals Co., Ltd.

Renesas Design (uk) Limited

Tianjin Asymchem Pharmaceutical Co., Ltd.

Asymchem Life Sciences (tianjin) Co., Ltd.

Asymchem Life Science Co., Ltd.

Yanjun Li

Hao Hong

Jiangping Lu

Na Zhang

Gage James

Kejian Zhang

Yulei Ma

Wenyan Yu

Fang Liu

Xin Huang

Qingyang Xu

Juan Gao

Junlu Wei

Chenglin Zhang

Hao Xu

Yan Zhang

Ning Chen

Xixian Xie

Xiaoguang Fan

Feng Gao

Heyun Wu

Qian Ma

Yu Zhang

Qiang Li

Yanzhao Wang

Youzhong Liu

Yixuan Ma

Shaohe Li

Shibao Han

Guoliang Li

Nan Lu

Yingzi Li

Ziyi Li

Fuzhou Zhu

Zishen Wang

Xierong Dong

A regulator circuit is presented. The regulation circuit () has a regulation switch (), a voltage comparator () with predetermined offset voltage and an adjuster. The regulation switch () has a first terminal coupled to a current source (), a second terminal connectable to a load (), and a control terminal. The voltage comparator () compares a sum voltage of the offset voltage added to a voltage at the second terminal with a control voltage at the control terminal, and generates a comparison signal. The adjuster adjusts the voltage at the second terminal based on the comparison signal to control a difference voltage between the control terminal and the second terminal of the regulation switch and to maintain the regulation switch operating in a desired region of operation.

Regulator circuit

The invention relates to a 2-isopropyl malate synthase, a genetically engineered bacterium for producing L-leucine and application thereof and belongs to the field of metabolic engineering. The genetically engineered bacterium is obtained by overexpressing an isopropyl malate synthase coding gene leuAfor relieving feedback inhibition by L-leucine, an acetohydroxy acid synthase coding gene ilvBNfor relieving feedback inhibition by L-isoleucine, a 3-isopropyl malate dehydrogenase coding gene leuB and a 3-isopropyl malate dehydratase coding gene leuCD in host cells. The genetically engineered bacterium for producing the L-leucine is free from nutritional deficiency, rapid in growth, short in fermentation period, high in yield and high in conversion rate.

2-isopropylmalate synthetase and engineering bacteria and application thereof

Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity.

Proline hydroxylase and uses thereof

The present disclosure relates to a genetically engineered strain with high production of uridine and its construction method and application. The strain was constructed as follows: heterologously expressing pyrimidine nucleoside operon sequence pyrBCAKDFE (SEQ ID NO:1) on the genome ofprompted by strong promoter Pto reconstruct the pathway of uridine synthesis; overexpressing the autologous prsA gene coding PRPP synthase by integration of another copy of prsA gene promoted by strong promoter Pon the genome; deficiency of uridine kinase, uridine phosphorylase, ribonucleoside hydrolase, homoserine dehydrogenase I and ornithine carbamoyltransferase. When the bacteria was used for producing uridine, 40-67 g/L uridine could be obtained in a 5 L fermentor after fermentation for 40-70 h using the technical scheme provided by the disclosure with the maximum productivity of 0.15-0.25 g uridine/g glucose and 1.5 g/L/h respectively which is the highest level of fermentative producing uridine reported at present.

Genetically engineered bacterium used for producing uridine with high-yield

Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein having an amino acid sequence of SEQ HD NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity; or (c) a protein retaining the mutation of one or more amino acids as in (b), and having the proline hydroxylase activity and having at least 78% homology with the amino acid sequence of the protein in (b). Protein having the amino acid sequence as shown in SEQ HD NO: 2 and mutants obtained by genetically engineering have higher catalytic specificity or significantly increased catalytic activity when compared to proline hydroxylases in prior art.

A transaminase and a use thereof are provided. The transaminase has the amino acid sequences as shown in SEQ ID NO: 2 or 4, or has at least 80% identity to the amino acid sequences as shown in SEQ ID NO: 2 or 4, or has amino acid sequences which are obtained by the substitution, deletion or addition of one or more amino acids and have an the activity of an omega-transaminase with high stereoselective R-configuration catalytic activity, wherein the high stereoselective refers to the content of one of the stereoisomers being at least about 1.1 times that of the other.

Transaminase and uses thereof

Growing community of inventors

Tianjin, China

Yanjun Li