Zhejiang Academy of Agricultural Sciences

Chinese Academy of Agricultural Sciences

Yanhua Wang

Qiang Wang

Guiling Yang

Chen Chen

Xinquan Wang

Zhiheng Zhang

Shanshan Di

Yongzhong Qian

The present invention provides a test method for toxicity of raw dinotefuran drug on early life stage of fish. The method includes: preparing a standard diluent, preparing test drug liquids, selecting a test organism, toxicant exposure of the embryos, toxicant exposure of larvae, monitoring and controlling test conditions, observation in the test, and statistically analyzing data and drawing results.

Test method for toxicity of raw dinotefuran drug on early life stage of fish

A device and a method are disclosed for collecting and culturing fish embryos and evaluating combined toxicity of thiamethoxam and tetraconazole. An embryo collection component and an embryo culture component are provided on a base plate. The embryo collection component includes a collection tray detachably connected to a bottom of a culture tube. The embryo culture component includes a cavity plate with a stopper inside and a top plate provided with a culture well. A bottom of the culture well is provided with a leakage hole; and after the top plate is installed into the cavity plate, the stopper occludes the leakage hole. The new device is used to carry out the combined toxicity effect test of thiamethoxam and tetraconazole on zebrafish embryos, which can be used to avoid development of pesticide mixtures that have a good preventive effect but pose increased toxicity risk to the ecological environment.

Device for collecting and culturing fish embryos and method for evaluating combined toxicity of thiamethoxam and tetraconazole

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Hangzhou, China

Yanhua Wang