Chinese Academy of Sciences

Cofco Biochemical (anhui) Co., Ltd.

Cofco Biochemical Co., Ltd

Yanhe Ma

Tao Sun

Jibin Sun

Ping Zheng

Jing Min Xu

Qian Jack Liu

Xueli Zhang

Chaoguang Tian

Hongtao Xu

Jiao Liu

Jingen Li

Jinlei Tang

Jiangang Yang

Wenliang Sun

Yunjie Li

Liangcai Lin

Chuannan Long

Yuanxia Sun

Jingxiao Ji

Jiuzhou Chen

Tuo Shi

Weidong Liu

Jing Zhao

Chun You

Xinna Zhu

Wenxia Jiang

Wei Pu

Pingping Liu

Guojun Yue

Qinggang Li

Linghe Xia

Xinyang Wu

Hu Luo

Yong Zhou

Yun Man

Yongsheng Zhou

Xiaoran Zhang

Rey-Ting Guo

Qingyan Li

Yingying Zheng

Guanping Dai

Zongmei Lu

Xuan Guo

Wenjuan Zhou

Zhongxia Xu

Yueming Zhu

Chun-Chi Chen

Wei Peng

Je-Ruei Liu

Deyong Ge

Yanfen Xue

Jing Xu

Qian Liu

Provided arethat produces L-lysine, a method for constructing the L-lysine producing strain, and a method for producing L-lysine from the strain. The lysine yield and glucose conversion of the L-lysine producing strain are improved, thereby reducing the production cost.

Method for producing lysine from microorganism and for constructing microorganism

Recombinant strains are obtained for the production of allulose, allose, and allitol by regulating intracellular glucose metabolism, reducing the enzyme activity of fructose 6-phosphate kinase, and enhancing the enzyme activities of glucokinase and glucose-6-phosphate isomerase, allulose 6-phosphate 3-epimerase, allulose 6-phosphate phosphatase, fructose permease and fructokinase, and optionally enhancing the enzyme activities of ribose 5-phosphate isomerase, allose 6-phosphate phosphatase, ribitol dehydrogenase, glycerol permease, glycerol dehydrogenase, and dihydroxyacetone kinase. A method for producing allulose and allose is an extracellular multienzyme cascade method. Multienzyme cascade catalysis and fermentation are coupled to improve the conversion rate of starch sugar or sucrose to the synthesized allulose.

Engineered strain for producing allulose and derivatives thereof, method for construction therefor and use thereof

Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved. The dibasic organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; the expression product of the positive regulator gene comprises aspartate aminotransferase, glutamic acid-aspartate transporter, C4-dicarboxylic acid transporter, pyruvate carboxylase and malate dehydrogenase, glucose transporter; the expression product of the negative regulatory gene comprises succinyl-CoA synthase, and malic acid-alpha ketoglutarate transporter, and the original strain comprises, and

Dibasic organic acid producing strain and preparation and application of same

Disclosed is a new tagatose 6-phosphate 4-epimerase, which is capable of converting fructose 6-phosphate into tagatose 6-phosphate and vice versa. Also disclosed is an application of the enzyme in tagatose production.

Tagatose-6-phosphate 4-epimerase and application thereof

A method for constructing an ALA production bacterial strain, the method enhances the activity of related enzymes promoting the synthesis of oxaloacetate and in the 5-aminolevulinic acid (ALA) production bacterial strain, or introducing exogenous related enzymes promoting the synthesis of oxaloacetate, such as phosphoenolpyruvate carboxylase or pyruvate carboxylase, and/or reducing the activity of related enzymes in the downstream metabolic pathway of succinyl coenzyme A in the bacterial strain, such as succinyl coenzyme A synthetase or succinate dehydrogenase, and/or reducing the activity of phosphoenolpyruvate carboxylated kinase and/or malic enzyme. An ALA high-yield bacterial strain constructed by utilizing the method, and method for utilizing the bacterial strain to prepare ALA.

5-aminolevulinic acid high-yield bacterial strain, preparation method and use thereof

Disclosed is a solid state biological reaction device, comprising a main tank (), wherein the device further comprises a support () supporting the main tank (), and the support () makes the main tank () be rotational in the horizontal position, and be statically cultured in the vertical position. The device is relatively simple, in particular, the mixing of materials uses the method of a vehicle-tank in combination with rotation, achieving the tank free conversion between the two different poses of vertical and the horizontal; and the device conducts the work of loading, inoculation, cultivation and transplantation and so on in the upright pose, and completes the work of sterilization and mixing of materials and so on in the horizontal pose. The device is not only quick to use and easy to move, but also omits the stirring system which occupies a lot of manufacturing costs, and is easy to use in large-scale production.

Solid state biological reaction device, usage method and use thereof

A lactone hydrolase having improved activity towards &#x3b1;-zearalenol is disclosed. The lactone hydrolase has a modified amino acid sequence of SEQ ID NO: 5, wherein the modification is a substitution of valine at position 167 with histidine. A method of degrading &#x3b1;-zearalenol using such lactone hydrolase is also disclosed.

Lactone hydrolase and method of degrading alpha-zearalenol using the same

The invention provides a polypeptide capable of using 4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP) as a substrate to produce isoprene, a nucleic acid encoding the polypeptide, and a vector and a cell comprising the nucleic acid. In addition, the invention also provides a method for producing isoprene using the polypeptide, and a method for preparing the polypeptide.

Enzyme for biosynthesis of isoprene and isopentenyl, and mutant thereof

Provided is recombinantcomprising a mutated ldhA gene. Disclosed are use of the recombinantin the preparation of D-lactate, and method of preparing D-lactate by employing the recombinant. In the method of preparing D-lactate, fermentation can be conducted at a high temperature (42-50&#xb0; C.), and aqueous ammonia or sodium hydroxide can be used as a neutralizer during the fermentation.

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Provided in the present invention is an asparaginic acid kinase, and the 340amino acid residue in the position of the amino acid sequence of the asparaginic acid kinase corresponding to the amino acid sequence shown in SEQ ID NO: 2 is a non-aspartic acid. The asparaginic acid of the present invention can efficiently relieve the feedback inhibition of L-lysine, and can be effectively used for the production of L-lysine. Also provided in the present invention are host cells comprising genes coding the asparaginic acid kinase and a method for producing L-lysine using the host cells or the asparaginic acid kinase. The asparaginic acid kinase of the present invention or the host cells comprising the asparaginic acid kinase of the present invention is also used to produce L-threonine, L-methionine, L-isoleucine or L-valine. Also provided in the present invention is a method of producing L-aspartyl-4-yl phosphoric acid using the asparaginic acid kinase or the host cells.

Asparaginic acid kinase III mutant and host cells and use thereof

Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of &#x3b1;-ketoglutarate dehydrogenase inor the mutant thereof. The method for improving the enzymatic activity of &#x3b1;-ketoglutarate dehydrogenase inor the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) inor the mutant thereof with any of the following regulating elements: artificial regulating element M1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria. By improving the enzymatic activity of &#x3b1;-ketoglutarate dehydrogenase, succinic acid dehydrogenase and transaldolase therein and improving the ability of a cell to synthesize NADPH and ATP, the efficiency of the MEP pathway and the production capacity of terpenoid are improved.

Recombinant microorganism for preparing terpenoid and method for constructing recombinant microorganism

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Tianjin, China

Yanhe Ma