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Feed Research Institute Chinese Academy of Agricut

Yangyang Li

Bin Yao

Yaru Wang

Huoqing Huang

Huiying Luo

Peilong Yang

Canfang Niu

Dali Yu

Yongkai Du

The present invention relates to the field of genetic engineering, particularly to phytase variant YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency, by substituting Leucine at the 162site of the sequence set forth in SEQ ID NO.1 with glycine or proline or substituting glutamic acid at the 230site of the sequence set forth in SEQ ID NO.1 with glycine, proline or arginine, in the benefit of the development of economical feed enzyme industry.

Phytase variants YeAPPA having improved gastric protein resistance and acid resistance, and increased catalytic efficiency

The present invention relates to the field of genetic engineering, particularly to phytase variants YkAPPA having amino acid sequence substituting Leucine at the 162site of the sequence set forth in SEQ ID NO.1 with glycine or proline, or having amino acid sequence substituting glutamic acid at the 230site of the sequence set forth in SEQ ID NO.1 with glycine, alanine, serine, threonine, aspartic acid, proline, or arginine, and having improved pepsin resistance and increased catalytic efficiency of 2.1 times of that of the wild phytase, in the benefit of the development of economical feed enzyme industry.

Phytase variants YkAPPA having improved pepsin resistance and increased catalytic efficiency

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Beijing, China

Yangyang Li