National Research Council of Canada

Yangdou Wei

Jitao Zou

David Charles Taylor

Colette C Jako

Gopalan Selvaraj

Raju S Datla

Cyril Periappuram

David C Taylor

The present invention relates to the isolation, purification, characterization and use of the plant diacylglycerol acyltransferase (DGAT) gene and genetic products. The invention includes isolated and purified DGAT DNA and relates to methods of regulating seed oil content, the ratio of diacylglycerol/triacylglycerol proportions in the seed oil, fatty acid synthesis, seed oil acryl composition, seed size/weight and carbon flux into other seed components, using the gene, and to tissues and plants transformed with the gene. The invention also relates to transgenic plants, plant tissues and plant seeds having a genome containing an introduced DNA sequence of the invention, and a method of producing such plants and plant seeds.

Diacylglycerol acyltransferase gene from plants

The present invention relates to the isolation, purification, characterization and use of the plant diacylglycerol acyltransferase (DGAT) gene and genetic products. The invention includes isolated and purified DGAT DNA and relates to methods of regulating seed oil content, the ratio of diacylglycerol/triacylglycerol proportions in the seed oil, fatty acid synthesis, seed oil acyl composition, seed size/weight and carbon flux into other seed components, using the gene, and to tissues and plants transformed with the gene. The invention also relates to transgenic plants, plant tissues and plant seeds having a genome containing an introduced DNA sequence of the invention, and a method of producing such plants and plant seeds.

The invention provides a method for genetically transforming a plant so that it expresses a glycerol-3-phosphate dehydrogenase that is feedback-defective. The feedback-defective enzyme raises levels of glycerol and glycerol-3-phosphate in comparison to the wild-type, leading to increased osmotic stress tolerance, and altered fatty acid content in glycerolipids.

Transgenic manipulation of sn-glycerol-3-phosphate and glycerol production with a feedback defective glycerol-3-phosphate dehydrogenese gene

The present invention relates to the isolation, purification, characterization and use of the plant diacylglycerol acyltransferase, DGAT, gene and associated gene products. The invention includes isolated and purified DGAT cDNA (SEQ ID NO: 1) and a plant diacylglycerol acyltransferase gene (SEQ ID NO: 3) and homologues thereof from the, such as. Methods of using the DGAT DNA to regulate seed oil content, the ratio of diacylglycerol/triacylglycerol proportions in the seed oil, fatty acid synthesis, seed oil acyl composition, seed size/weight and carbon flux into other seed components are also included. Methods of producing transgenic plants, plant tissues and plant seeds having genomes including DGAT genes, and the resultant transgenic plants, plant tissues and plant seeds are also included.

Growing community of inventors

Saskatoon, Canada

Yangdou Wei